APP17肽对2型糖尿病KK小鼠海马神经元超微结构和InsR、IRS-1的影响  

Effect of APP17 Peptide on the Hippocampal Neurons Ultrastructure and Expression of InsR, Ins-1 in Type 2 Diabetic KKAy Mice

作　　者：庄晓明[1] 赵志炜[2] 姬志娟[2] 赵咏梅[2] 盛树力[2] 

机构地区：[1]首都医科大学附属北京复兴医院内分泌科 [2]首都医科大学宣武医院

出　　处：《首都医科大学学报》2002年第2期115-118,共4页Journal of Capital Medical University

基　　金：国家科技部 973课题资助项目 (G2 0 0 0 0 5 70 10 )

摘　　要：为观察APP1 7肽对糖尿病KKAy小鼠 (以下简称KK小鼠 )脑海马神经元部分信号转导蛋白表达的影响 ,并探讨APP1 7肽对神经元的营养作用 ,将KK小鼠分为正常对照组 (C组 )、糖尿病组 (DM组 )和APP1 7肽治疗组(DM +APP1 7P组 ,给予APP1 7肽皮下注射 )。 1 2周后将 3组小鼠处死 ,心脏取血测血糖和血浆胰岛素 ;灌注固定后 ,取海马送电镜检查并作免疫组织化学染色。结果显示 :①DM组与DM +APP1 7P组的血糖、胰岛素水平比C组显著升高 (P <0 .0 5 ) ,但是DM组与DM +APP1 7P组之间无显著差异 ;②DM组海马神经元胰岛素受体 (InsR)、胰岛素受体底物 1 (IRS 1 )的表达低于C组和DM +APP1 7P组 (P <0 .0 5 ) ;③海马超微结构显示DM组海马神经元线粒体肿胀 ,未见凋亡的神经元 ,DM +APP1 7P组和C组神经元基本正常。提示 :APP1 7肽作为神经营养因子能激活信息转导通路 ,从而对神经元凋亡有改善作用。The objective of the study was to investigate the expression of apoptotic-related proteins in hippocampal neurons of KK mice and the hippocampus ultrastructure in diabetic KK mice. KK mice were divided into three groups: normal control group, diabetic group and diabetic group treated with APP17-mer peptide. After 12 weeks, crystat sections of mouse brain were prepared and immunohistochemically stained for InsR, IRS-1. Three groups of the mice were observed and analyzed by electron microscopy. The levels of blood glucose and plasma insulin in DM group and DM+APP17P group were significantly increased and compared with C group. There were no significant difference between DM group and DM+ APP17P group. The expression of InsR, IRS-1 significantly decreased in diabetic mice as compared with normal controls (P<0.05), and find mitochondria edema in hippocampus in DM group. APP17 peptide may activate the signal transductive pathway. It may also improve the apoptosis of neuron in type 2 diabetic KK mice.

关 键 词：APP17肽 2型糖尿病 信号转导通路 神经元超微结构 动物实验 海马区 INSR IRS-1 

分 类 号：R587.2[医药卫生—内分泌]