心脏移植后外周血FK506结合蛋白1A基因的表达  

Gene Expression of Peripheral Blood from a Heart Graft Patient

作　　者：许秀芳[1] 李温斌[2] 孟旭[2] 李文[1] 刘艳霞[1] 刘舒[1] 周健[3] 黄益民[1] 

机构地区：[1]北京市心肺血管疾病研究所细胞免疫室 [2]北京安贞医院心外科 [3]中科院微生物研究所

出　　处：《首都医科大学学报》2002年第2期125-127,共3页Journal of Capital Medical University

基　　金：北京市心血管病研究实验室基金资助 ;北京市卫生局重点学科基金资助项目

摘　　要：利用荧光差异显示PCR(DD PCR)技术检测心脏移植后外周血白细胞基因的表达。结果显示 :RPL2 3基因移植后有波动 ,排斥发生时表达增强 ;FK5 0 6结合蛋白 1A基因移植后表达较弱 ,排斥发生时明显增强。提示 :外周血DD PCR检测白细胞基因的表达可能对研究排斥反应机制及进行移植排斥反应诊断具有一定的意义。In the paper the technique known as differential display polymerase chain reaction(DD-PCR) was applied to explore genes expression in white cells of peripheral blood drawn from an allogenic heart graft patient on the day before the transplant operation and after the operation in order to assess genes′role in transplantation. Morphologic changes of the heart grafts were observed 11 d after transplant operation. RPL23 gene was expressed undulatority after the operation, but strongly when acute rejection occured; FK506 binding protein 1A gene was expressed weakly after the operation,but strongly when acute rejection occured. The result indicates that the study of genes expression of peripheral blood from a heart graft patient will be useful to assess genes′role in transplantation

关 键 词：心脏移植 外周血 FK506结合蛋白1A基因 免疫排斥 荧光差异显示PCR 

分 类 号：R654.2[医药卫生—外科学]