地衣芽孢杆菌NK-27菌株β-甘露糖苷酶的产酶条件及粗酶性质  

STUDIES ON THE PRODUCTION OF β-MANNOSIDASE BY BACILLUS LICHENIFORMIS NK-27

作　　者：杨先芹[1] 孙丹[1] 杨文博[1] 白钢[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》2002年第2期117-120,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：地衣芽孢杆菌 NK-2 7菌株可以产生胞外β-甘露聚糖酶和胞内β-甘露糖苷酶 .β-甘露糖苷酶活力主要集中于细胞匀浆液中 .以 2 .0 %魔芋粉、1 .0 % ( NH4) 2 SO4为碳、氮源地衣芽孢杆菌 NK-2 7菌株经 30℃振荡培养 2 0～2 4 h产生 β-甘露糖苷酶酶活力最高 .该酶于 30～ 4 0℃、p H6 .0时酶活力最高 ,在 30～ 4 0℃、p H5 .4～ 7.Bacillus Licheniformis NK-27 is capable of producing extracellular β-mannanase and intracellular β-mannosidase. β-mannosidase could be efficiently got in the medium composed of 2.0% konjac powder, 1.0% (NH 4) 2SO 4 and 0.2% NaCO 3 after cultivating 20～24 hours at 30℃. The optimal temperature and pH for β-mannosidase reaction were 30～40℃ and pH 7.0. The activity of β-mannosidase was concentrated at the cell homogenate of Bacillus Licheniformis NK-27.

关 键 词：β-甘露糖苷酶 地衣芽孢杆菌NK-27菌株 粗酶性质 

分 类 号：Q814.1[生物学—生物工程]