Effects of Coriolus versicolor polysaccharide B on monocyte chemoattractant protein 1 gene expression in rat  

云芝多糖B对大鼠单核细胞趋化蛋白-1基因表达的影响(英文)

作　　者：宋烈昌 陈海生[1] 娄宁[2] 宋畅[3] 曾钧[2] 傅庭焕[2] 

机构地区：[1]第二军医大学药学院,上海200433 [2]第一军医大学卫生学系分子药理实验室,广州510515 [3]中山大学中山医学院生化教研室,广州中国510089

出　　处：《Acta Pharmacologica Sinica》2002年第6期539-543,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 39700177.

摘　　要：AIM: To investigate the effect of Coriolus versicolor polysaccharide B (CVPS-B), a new water-soluble component of polysaccharides from the fungus Coriolus versicolor (Fr) L on monocyte chemoattractant protein-] ( MCP-1 ) gene expression in rat splenocytes. METHODS: Expression of MCP-1 mRNA in rat splenocytes was examined by reverse transcription-polymerase chain reaction (RT-PCR) with β-actin as an internal standard. Sequencing of RT-PCR products was performed to confirm their specificity in MCP-1 gene composition. RESULTS: (1) Without pre-treatment of lipopolysaccharide (LPS), the relative MCP-1 mRNA expression ratios (MCP-1/β-actin) for the saline control group and for CVPS-B groups in 3 different doses (10, 20, and 30mg·kg-1·d-1, ip, for4 d) were 1.4±0.3, 1.6± 0.4, 1.7±0.5, and 1.5 ± 0.4, respectively (P>0.05); (2) LPS (10μg·kg-1, ip) enhanced the expression of MPC-1 mRNA by the ratio of 114 % ; (3) pre-treatment with CVPS-B of 4 different doses (5, 10, 30, and 50 mg·kg-1 ·d-1, ip, for 4 d) decreased the LPS induced expression of MPC-1 mRNA by the ratios of 51 % , 70 % , 84 % , and 99 % , respectively (n = 6). CONCLUSION: In a dose-related fashion, CVPS-B inhibited the expression of MCP-1 mRNA induced by LPS in the rat splenocytes, but did not significantly affect the expression of MPC-1 mRNA in the normal rat.目的:探究野生云芝多糖水溶性新组分CVPS-B对大鼠脾细胞单核细胞趋化蛋白-1(MCP-1)基因表达的影响.方法:以β-actin为内标准物,用逆转录聚合酶链式反应(RT-PCR)检测CVPS-B分别对正常情况下以及脂多糖(LPS)诱导下大鼠脾细胞MCP-I基因表达的影响,并对RT-PCR产物进行测序,以证实其特异性.结果:(1)正常情况下大鼠脾细胞MCP-1 mRNA的表达(MCP-1/β-actin的比值)生理盐水对照组为1.4±0.3;CVPS-B三个剂量组(10、30和50mg·kg^(-1)·d^(-1),ip,连续4d)分别为:1.6±0.4、1.7±0.5和1.5±0.4,后三组与对照组无显著差异(P>0.05);(2)大鼠腹腔给药LPS(10μg·kg^(-1)可使脾细胞MCP-1 mRNA的表达增加114％.(3)CVPS-B4个剂量组(5、10、30和50mg·kg^(-1)·d^(-1),ip,连续4d)可使LPS(10μg·kg^(-1),ip)诱导的脾细胞MCP-1 mRNA的表达分别减低51％,70％,84％和99％(n=6).结论:CVPS-B可预防性抑制LPS对大鼠脾细胞MCP-1基因表达的诱导作用,且呈剂量依赖性,但对正常情况下大鼠脾细胞MCP-1 mRNA的表达则无明显影响.

关 键 词：Coriolus versicolor polysacchandes monocyte chemoattractant protein-1 gene expression RATS 

分 类 号：R285[医药卫生—中药学] R329.28[医药卫生—中医学]