“微岛”原代培养新生大鼠中脑腹侧多巴胺能神经元  

Microisland" Primary Culture of Dopaminergic Neuron from Postnatal Rat Ventral Midbrain

作　　者：郑志竑[1] 林玲[1] 胡建石[1] 朱进伟[1] 林建银[1] 

机构地区：[1]福建医科大学分子医学研究中心,福州350004

出　　处：《福建医科大学学报》2002年第2期123-126,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目 (C970 3 8)

摘　　要：目的　探讨“微岛”原代培养中脑腹侧多巴胺能神经元的方法 ,研究不同培养液维持多巴胺能神经元存活和生长的效果 ,及多巴胺能神经元与其他神经元在“微岛”上的共培养 ,为多巴胺能神经元的相关研究提供一种新的细胞模型。　方法　利用神经细胞不能粘附于琼脂糖上生长的原理 ,先用琼脂糖制成非粘附性底物 ,再将胶原喷洒其上形成粘附性的“岛”样底物 ,接种神经胶质细胞形成微岛样饲养层后 ,将大鼠中脑腹侧神经元接种于“微岛”上生长。　结果　中脑腹侧多巴胺能神经元可在“微岛”上生长 ,即使单个神经元也生长良好。在共培养时 ,能与其他神经元形成突触联系。　结论　确定适于“微岛”原代培养中脑腹侧多巴胺能神经元的培养条件 。Objective\ To explore an optimal 'microisland' primary culture method for dopaminergic neuron from postnatal rat ventral midbrain, and to investigate the effects of different medium for maintaining neuron survival and growth.\ Methods\ 'Microisland' culture technique used agarose as a substrate that was virtually non\|adhesive for glial cells, then droplets of collagen were applied to this non\|adhesive surface in order to provide a consistent and uniform substrate for glial attachment.\ Glial feeder layers were estabilished and then the neurons from postnatal rat midbrain were cultured on the 'microisland'.\ Results\ 'Microislands' made one or several dopaminergic neurons growing in a small area, isolated from other neurons.\ A much higher frequency of synaptic connection could be achieved in 'microisland' co\|cultures.\ Conclusion\ 'Microisland' culture medium of dopaminergic neuron from ventral midbrain are established.\ It can be a new cellular model for studying dopaminergic neuron.\;

关 键 词：多巴胺 神经元 中脑 细胞 培养的 大鼠 

分 类 号：Q42[生物学—神经生物学]