胰岛素对NIH3T3细胞生长和Ras-MAPK信号途径的影响  

Effects of Insulin on Proliferation of NIH3T3 Cells and Ras-MAPK Signal Transduction Pathway

作　　者：何艳[1] 叶庆林[1] 林德馨[1] 高永琳[1] 

机构地区：[1]福建医科大学生物化学与分子生物学系,福州350004

出　　处：《福建医科大学学报》2002年第2期130-133,共4页Journal of Fujian Medical University

基　　金：福建省教育厅科研基金资助项目 (JA99183 )

摘　　要：目的　探讨胰岛素激活的 Ras- MAPK信号途径对鼠成纤维细胞 (NIH3T3细胞 )生长的影响。　方法　藉四唑蓝比色法 (MTT法 )和蛋白免疫印迹试验分别观察胰岛素对 NIH3T3细胞生长和胞内丝裂原激活蛋白激酶 (MAPK)活性的影响。　结果　胰岛素对 NIH3T3细胞的促增殖作用呈时间和浓度依赖性 ,10 m U/ m l胰岛素对NIH3T3细胞的促增殖作用效果最佳 ,对 NIH3T3细胞作用的 EC50 是 0 .2 m U/ m l(48h) ;0 .14 m U/ ml(72 h)。MAPK在 10 m U/ ml胰岛素刺激 1min后即出现酪氨酸磷酸化 ,10 min达到高峰。胰岛素作用 10 min,MAPK酪氨酸磷酸化程度随浓度增高 (0 .1,1.0 ,10 m U/ ml)而递增。　结论　胰岛素对 NIH3T3细胞促增殖作用与激活 Ras-MAPK信号途径相关。Objective\ To study the effect of Ras\|MAPK signal transduction pathway activated by insulin on proliferation of mouse fibroblasts(NIH3T3 cells).\ Methods\ The growth and activation of mitogen\|activated protein kinase(MAPK) of NIH3T3 cells treated with insulin were investigated with MTT cell count method and western\|blotting methods respectively.\ Results\ Insulin enhanced the proliferation of NIH3T3 cells, depending on its dose and time used.\ 10 mU/ml insulin was the best dose.\ The EC\-\{50\} of insulin on NIH3T3 cells was 0 2 mU/ml and 0 14 mU/ml at 48 hours and 72 hours respectively.\ \{Insulin\} induced the activity of tyrosine phosphorylation of MAPK rapidly, and was detectable at 1 min, reached maximum at 10 min, and the degree of tyrosine phosphorylation of MAPK was higher at 10 min when the dose of insulin increased from 0 1 to 10 mU/ml.\ Conclusion\ The effect of insulin on proliferation of NIH3T3 cells relates to Ras\|MAPK signal transduction pathway. \;

关 键 词：信号传递 胰岛素 蛋白激酶类 有丝分裂原 

分 类 号：R965[医药卫生—药理学]