致肾盂肾炎大肠杆菌P菌毛粘附素基因的型别鉴定  

Identification of Variants of P pili Adhesin Gene of Uropathogenic Escherichia coli

作　　者：郑铃[1] 陈锦英[2] 陈豪[3] 陈贻锴[1] 詹丽钦[1] 

机构地区：[1]福建医科大学基因工程研究室,福州350004 [2]天津医科大学微生物学教研室,天津300070 [3]福建医科大学寄生虫学教研室,福州350004

出　　处：《福建医科大学学报》2002年第2期137-140,共4页Journal of Fujian Medical University

摘　　要：目的　从基因水平鉴定致肾盂肾炎大肠杆菌 P菌毛粘附素的类型。　方法　根据 P菌毛 型(Pap GJ96 )、 型 (Pap GIA2 )和 型 (Prs GJ96 )粘附素的基因序列 ,选择非同源区设计合成三对引物。以全菌 DNA样品为模板 ,应用普通 PCR和复合 PCR技术获得三型 pap G的扩增。　结果　 U PEC J96分别产生 4 6 1bp和 2 5 8bp的DNA片段 ;U PEC132和 U PEC136均产生 190 bp的 DNA片段。无 P菌毛对照株为阴性扩增。复合 PCR结果与普通 PCR完全一致。Pap G1 32 扩增产物的序列分析表明其与 Pap GIA2 之间具有 97.9%的同源性 ,但存在 4处点突变。　结论　 U PEC132株 P菌毛粘附素为 型 Pap G,但与国外同型 Pap G比较 ,存在基因变异。复合 PCR技术用于papObjective\ To identify three recognized gene variants(Ⅰ to Ⅲ) of P pili adhesin gene papG among uropathogenic Escherichia coli strains.\ Methods\ Primer pairs specific for each of the three papG classes were designed on the basis of DNA sequences of PapG J96, PapGIA2 and PrsG J96.\ Amplifications of the three allelies of papG were obtained by singly and multiply primed papG PCR.\ \{Results\}\ Two kinds of amplifications(461 bp and 258 bp) from UPEC J96 containing classes Ⅰ and Ⅲ papG and \{another\} kind(190 bp) from both UPEC132 and UPEC136 strains but none from control strain with P pili absence were identified by above PCR methods.\ Sequence analysis of the product from UPEC132 showed that it contained a class Ⅱ PapG which shared 97 9% homology with that from \{UPECIA2\}, but there were four mutations in it.\ Conclusion\ The adhesin of P pili derived from \{UPEC132\} is a novel variant of papG allelie Ⅱ.\ The multiply primed PCR was a highly sensitive and specific method for identification of papG gene variants. \;

关 键 词：肾盂肾炎 大肠杆菌 基因 细菌 细胞分型技术 细菌粘附 

分 类 号：R691.3[医药卫生—泌尿科学]