大鼠不同类型臂丛根性损伤后脊髓运动神经元NOS的表达  

NOS Expression of Spinal Motoneurons after Different Brachial Plexus Root Injuries

作　　者：张立群[1] 张文明[1] 朱维钦[1] 林建华[1] 

机构地区：[1]福建医科大学附属第一医院骨科,福州350005

出　　处：《福建医科大学学报》2002年第2期157-160,F003,共5页Journal of Fujian Medical University

基　　金：福建省科技厅医药卫生科技攻关计划资助项目 (98-Z-166) ;福建省教育厅科研基金资助项目 (JB9914 4) )

摘　　要：目的　研究成年大鼠不同类型臂丛损伤后脊髓运动神经元一氧化氮合酶 (NOS)表达与神经元死亡之间的关系。　方法　选用 76只健康成年 SD大鼠 ,分别造成臂丛神经根性切断伤与根性撕脱伤 ,于损伤后不同时间观察脊髓前角运动神经元的存活率 ,以及 NOS在脊髓前角运动神经元中的表达情况。　结果　臂丛神经根性切断伤后 ,损伤侧脊髓前角运动神经元无明显死亡 ,且未见 NOS表达。臂丛神经根性撕脱伤后 1周 ,损伤侧脊髓前角运动神经元数目开始减少 ,并出现 NOS阳性表达。之后随着 NOS阳性神经元增多 ,神经元死亡率也增加 ,6周后神经元数目基本维持稳定而 NOS阳性神经元逐渐减少消失。　结论　臂丛神经根性撕脱伤后 ,NOS参与损伤后脊髓运动神经元死亡 ,或在运动神经元死亡中起重要作用。Objective\ To investigate the relationship of nitric oxide synthase(NOS) expression and motoneurons damage after different brachial plexus root injuries.\ Methods\ 76 adult healthy SD rats were performed as the model of brachial plexus root avulsion or root axotomy.\ The spinal region was dissected for motoneuron counting and the expression of NOS was analyzed 1, 3 d, 1, 2, 3, 4, 6, 9, 12 week respectively after injury.\ Results\ Cell damage and NOS expression of spinal motoneurons occurred at 1 week after root avulsion.\ The motoneuron damage increased accordingly with the increase of NOS expression.\ 6 weeks later the number of NOS positive motoneurons was obviously decreased with no more motoneurons loss.\ However, no significant motoneuron damage and no NOS\|positive motoneurons were observed after spinal root axotomy.\ Conclusion\ NOS expression has been induced after brachial plexus root avulsion and is coincident with cell damage of spinal motoneurons.\ That NO may play important role in motoneurons damage. \;

关 键 词：臂丛根性损伤 脊髓运动神经元 细胞死亡 一氧化氮合酶 大鼠 

分 类 号：R651.3[医药卫生—外科学] R338.1[医药卫生—临床医学]