镉金属硫蛋白灌胃小鼠后镉的细胞分布及毒性  

Effects of Cadmium-metallothionein on Cellular Distribution of Cadmium and its Relationship with Splenic Lymphocyte Toxicity in Mice

作　　者：林忠宁[1] 董胜璋[1] 林育纯[1] 余贵英[1] 蔡颖[1] 王充[1] 

机构地区：[1]中山大学公共卫生学院,广州510089

出　　处：《中国公共卫生》2002年第7期778-779,共2页Chinese Journal of Public Health

基　　金：卫生部基金资助课题

摘　　要：目的　探讨镉金属硫蛋白 (CdMT)灌胃小鼠染毒后镉的细胞分布及淋巴细胞毒性。方法　应用分离纯化的CdMT灌胃染毒小鼠 4周 ,检测血红细胞镉 (RBC Cd)和脾淋巴细胞镉 (sLC Cd)的含量 ,及脾T淋巴细胞增殖功能、T细胞亚群和DNA单链断裂 (DNA SSBs)。结果　RBC Cd和sLC Cd在CdMT染毒后明显增高 ;脾淋巴细胞毒性表现为增殖减低 ,T细胞亚群改变和DNA SSBs尾长增加 ,且与细胞内镉含量有明显相关。结论　经消化道途径接触CdMT可致镉的吸收和在淋巴细胞内分布 。Objective To Study the effects of exogenous cadmium metallothionein(CdMT)on the distribution of cadmium in cell and its correlationship with cytotoxicity.Methods BALB/c mice were administered with the purified CdMT and Apo MT daily for 4 weeks as tested objects.Cadmium in erythrocyte(RBC Cd) and splenic lymphocyte(sLC Cd),as well as the cytotoxic indexes of splenic lymphocyte were detected.And the correlationship between each index was analyzed.Results RBC Cd and sLC Cd were increased significantly in groups treated with various dose of CdMT.There were obvious suppression of T lymphocyte proliferation,changes of T subsets and increasement of DNA single strands breaks(DNA SSBs).Conclusion There were Cd 2+ absorption in erythrocyte and redistribution in splenic lymphocyte of treated mice,which correlated with its immune suppression and the DNA damage after administered with CdMT via gastrointestine.

关 键 词：镉 金属硫蛋白 吸收分布 免疫毒性 

分 类 号：Q355[生物学—遗传学] Q344