从鼠、螨(革螨、恙螨)和人中分离HV的基因分型研究  

Study on Genotype of HV from Mice、Mites and Patients

作　　者：张云[1] 朱进[1] 陶开华[1] 钱万红[1] 吴光华[1] 王敬军[2] 张家驹[2] 邢爱华[2] 

机构地区：[1]南京军区军事医学研究所,南京210002 [2]陕西省疾病预防控制中心

出　　处：《中国公共卫生》2002年第7期780-781,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金资助项目 (39970 653 ;30 1 70 82 5);江苏省自然科学基金资助项目(BK2 0 0 0 1 1 53);解放军总后勤部卫生部"十五"自然科学基金资助项目 (0 1MA0 30 )

摘　　要：目的　了解鼠、螨 (革螨、恙螨 )、人间HV基因型是否一致。方法　对从同一疫区鼠、螨、人中分离的HV进行毒力测定 ;单克隆抗体检测和多聚酶链式反应 (PCR)进行基因分型。结果　从同一疫区鼠、螨、人中所分离的HV毒力和单克隆抗体检测均有I型 (HV型 )反应特征。进一步用PCR扩增鉴定 ,表明从鼠、螨、人中所分离的HV其基因型一致。结论　从同一疫区鼠、螨 (革螨、恙螨 )、人中所分离的HV其基因型一致 ,并构成三者相互传播关系 。Objective To investigate relation of gentype among mice,mites and patients.Methods Toxicity of HV from mice,mites and patients which all came from endemic area were qualified,HV were typed by monoclone antibody and RT-PCR.Results The results showed that there were HTV type in HV from mice,mites and patients,and had same gene type deteted by RT-PCR.Conclusion It demonstrated antigenicity of HV from mice,mites and patients were similar,HV could transmit among them.It played an important role in preserving epidemic area.

关 键 词：黑线姬鼠 革螨 恙螨 汉坦病毒 HV 基因分型 

分 类 号：R513[医药卫生—内科学]