表达马立克氏病病毒gI基因重组鸡痘病毒的免疫原性  

IMMUNOGENICITY AND STABILITY OF RECOMBINANT FOWLPOX VIRUS EXPRESSION GLYCOPROTEIN I OF MAREK′S DISEASE VIRUS

作　　者：陈志琳[1] 崔治中[1] 秦爱建[1] 张志[2] 吉荣[1] 刘岳龙[1] 金文杰[1] 

机构地区：[1]扬州大学畜牧兽医学院动物医学系,江苏扬州225009 [2]山东农业大学动物科技学院预防医学系,山东泰安271018

出　　处：《扬州大学学报（农业与生命科学版）》2002年第2期10-12,16,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家高新技术发展计划项目(863101050302)

摘　　要：以鸡痘病毒为载体,构建含马立克氏病病毒(MDV)gI基因的重组鸡痘病毒(rFPV),并在鸡胚成纤维细胞(CEF)中表达gI基因,用重组FPV(命名为rFPV MDV648gI)感染CEF,结果显示:rFPV MDV648gI能在CEF中稳定复制。进一步用rFPV MDV648gI接种无特定病原(SPF)鸡皮下组织,结果表明:鸡对rFPV MDV648gI具有抗体反应性。Recombinant fowlpox virus (FPV) expression glycoprotein I of Marek′s disease virus (MDV) was constructed. The recombinant FPV was named as rFPVMDV648gI. When rFPVMDV648gI was used to infect CEF, it showed that the recombinant virus could replicate stably after 20 passages. Sera from the chickens immunized with rFPVMDV648gI showed positive to MDV infected cells by IFA.

关 键 词：马立克氏病病毒 重组鸡痘病毒 糖蛋白gI 免疫原性 

分 类 号：S852.659.5[农业科学—基础兽医学]