灰葡萄孢拮抗细菌的种类鉴定及生长条件研究  

IDENTIFICATION OF BACTERIAL SPECIES ANTAGONISTIC AGAINST BOTRYTIS CINEREA AND CONDITIONS FOR THEIR GROWTH

作　　者：童蕴慧[1] 纪兆林[1] 徐敬友[1] 陈夕军[1] 卢国新[1] 

机构地区：[1]扬州大学农学院植物保护系,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2002年第2期67-70,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省"十五"科技攻关项目(BE2001328)

摘　　要：经形态和生理生化鉴定,对灰葡萄孢(BotrytiscinereaPers.)拮抗能力强、防病效果好的3株细菌进行分类,W10为地衣芽孢杆菌[Bacilluslicheniformis(Weigmann)Chester],W3和Y2 11 1为多粘类芽孢杆菌[Paenibacilluspolymyxa(Praz mowski)Ashetal]。这2种对蔬菜灰霉病的高效拮抗菌为中国首次报道和应用。3株拮抗细菌的生长条件基本一致。最适培养时间为24～72h,最适生长温度是27～30℃,最适培养液pH值为7～8。Three strains of bacteria antagonistic highly against Botrytis cinerea Pers. were isolated from tomato leaves and soil. According to the morphological, physiological and biochemical features, the isolate W10 was identified as Bacillus licheniformis (Weigmann) Chester and the isolates W3 and Y2111 belonged to Paenibacillus polymyxa (Prazmowski) Ash et al. It was first reported that these bacterial species had antagonism to B.cinerea on the vegetable in China. The optimum culture time for antibiotic bacteria ranged from 24 to 72 hours, the optimum growth temperature was from 27 to 30℃ and the optimum growth pH values in the media was from 7 to 8.

关 键 词：种类鉴定 生长条件 灰葡萄孢 拮抗细菌 地衣芽孢杆菌 多粘类芽孢杆菌 灰霉病 蔬菜病害 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]