微柱高效液相色谱-质谱/质谱快速鉴定混合蛋白质新方法  

A New Technique to Identify Individual Protein in Complex by Microcolumn-HPLC-ESIMS/MS

作　　者：张素艳[1] 王静[1] 卞利萍[1] 许云敏[2] 王洪海[2] 杨芃原[1] 

机构地区：[1]复旦大学化学系,上海200433 [2]复旦大学遗传研究所,上海200433

出　　处：《高等学校化学学报》2002年第7期1246-1250,共5页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究专项经费 (批准号 :2 0 0 1CB5 10 2 );上海市科学技术委员会重点项目资助

摘　　要：发展了一种混合蛋白质快速鉴定的新方法 .将几种蛋白质的混合物于热变性后直接在溶液中酶解 ,利用微柱高效液相色谱 -离子阱串级质谱进行肽谱 /氨基酸序列分析 ,并结合 Mascot数据库搜索处理功能 。In this work the identification method for protein mixture is reported, in which several proteins were varified by HPLC ESIMS/MS after the whole complex proteins were directly digested in solution, instead of the routine 2 dimensional gel electrophoresis. The obtained data was then searched against NCBInr database by using MS/MS Ions Search(http://www.matrixscience.com), and a positive result was therefore obtained. With the simulated sequence spectra in database searching, a re identification method was developed for low score matched protein by sequence tag technique. As a typical example, Concanavalin A, a less soluble protein with a low matching score, was also successfully identified, which indicated the great potential of complex digestion in proteomic study.

关 键 词：混合蛋白质 鉴定 蛋白质组学 微柱高效液相色谱 离子阱质谱/质谱 序列分析 

分 类 号：Q51[生物学—生物化学] Q503