醋酸精氨酸对严重烧伤家兔免疫功能的影响及其量效关系  

Effects of arginine acetate on immune function in burned rabbits and its doseeffect relationship

作　　者：彭曦[1] 汪仕良[1] 尤忠义[1] 王裴[1] 周新[1] 刘静[1] 

机构地区：[1]第三军医大学附属西南医院烧伤研究所,重庆400038

出　　处：《中国危重病急救医学》2002年第7期404-406,共3页Chinese Critical Care Medicine

基　　金：国家重点基础研究发展规划基金资助项目(No.G19990 5 42 0 2 )

摘　　要：目的 :研究醋酸精氨酸对严重烧伤家兔免疫功能的影响及其量效关系。方法 :采用家兔 (6 4只 ) 30 %体表面积 度烧伤后灌喂醋酸精氨酸模型 ,5 2只存活兔随机分成 6组 :正常对照组 (8只 ) ,烧伤对照组 (9只 )及醋酸精氨酸喂养 2 .4 g· kg- 1 · d- 1 组 (9只 )、1.2 g· kg- 1 · d- 1 组 (9只 )、0 .6 g· kg- 1 · d- 1 组 (9只 )、0 .3g· kg- 1· d- 1组 (8只 )。烧伤对照组予 1.2 g· kg- 1· d- 1酪氨酸 ,观察给药 7日后不同剂量精氨酸对烧伤家兔免疫功能的影响。结果 :烧伤 7日后各组动物免疫功能均明显下降 ,淋巴细胞转化率、白细胞吞噬率及趋化指数、血浆免疫球蛋白、CD4 / CD8比值均不同程度下降。精氨酸 1.2 g· kg- 1 · d- 1 组和 2 .4 g· kg- 1 · d- 1 组各项免疫指标均明显优于烧伤对照组 ,0 .3g· kg- 1· d- 1组和 0 .6 g· kg- 1· d- 1组与烧伤对照组相比变化不明显 ,但 2 .4 g· kg- 1 · d- 1 组动物死亡率有所上升。结论 :严重烧伤家兔机体免疫功能下降 ,经肠道补充精氨酸1.2 g· kg- 1· d- 1和 2 .4 g· kg- 1· d- 1能有效提高伤后免疫功能 ,以 1.2 g· kg- 1· d- 1效果最好 。Objective:To investigate the effects of arginine acetate on immune function in severely burned rabbits and its doseeffect relationship.Methods:Sixtyfour rabbits were inflicted with a 30% total body surface area(TBSA) fullthickness flame burn.Fiftytwo survival rabbits were randomly divided into 6 groups.Nine of these animals served as burn controls.The control group consisted of 8 rabbits.The rest of the injured animals were give arginine acetate in different doses after the injury:0 3 g·kg -1 ·d -1 ( n =8), 0 6 g·kg -1 ·d -1 ( n =9 ),1 2 g·kg -1 ·d -1 ( n=9 ),and 2 4 g·kg -1 ·d -1 ( n =9).Several parameters pertaining to immune function were determined after 7 days of treatment.Results:Impairment of immune function was observed after severe burn injury.Lymphocyte transformation ratio,polymorphonuclear leukocyte(PMN) phagocytosis and chemotaxis ratios as well as the content of immunoglobulin were decreased significantly 7 days after the injury.Compared with burn controls,immunological functions were improved after the supplementation of arginine with the dosage of 1 2 g·kg -1 ·d -1 and 2 4 g·kg -1 ·d -1 .There were no obviously changes with supplement 0 3 g·kg -1 ·d -1 and 0 6 g·kg -1 ·d -1 groups.However,the mortality rate of burned rabbits was increased in 2 4 g·kg -1 ·d -1 group.Conclusions:Immune functions were impaired after severe burn injury.Arginine acetate supplementation could enhance immunological function and decrease mortality rate.The optimal dose was 1 2 g·kg -1 ·d -1 .

关 键 词：量效关系 醋酸精氨酸 免疫功能 烧伤 家兔 

分 类 号：R644.05[医药卫生—外科学] R977.4[医药卫生—临床医学]