Bax和Bcl-2基因在单纯和放射复合伤口中的表达及与细胞凋亡和愈合延迟的关系  

Relationship between the expressions of Bax and Bcl2 genes and apoptosis and delayed wound healing in simple and radiation combined wound in rats

作　　者：崔玉芳[1] 夏国伟[1] 付小兵[2] 杨红[1] 彭瑞云[1] 谷庆阳[1] 高亚兵[1] 崔雪梅[1] 胡文华[1] 王小民[1] 王德文[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100050 [2]解放军第三○四医院创伤研究中心

出　　处：《中国危重病急救医学》2002年第7期421-423,共3页Chinese Critical Care Medicine

基　　金：全军"九.五"科研基金资助项目 (No.98Q0 86) ;国家"973"重大科研基金资助项目 (No.G19990 5 42 0 4)

摘　　要：目的 :研究 Bax、Bcl 2基因在单纯和放射复合伤口愈合中的表达规律及与细胞凋亡和愈合延迟的关系。方法 :用原位末端标记法 (TU NEL )检测细胞凋亡 ,用核酸原位杂交方法检测 Bax、Bcl 2 m RNA表达并定量分析。结果 :1照射后细胞凋亡出现的频度及延续的时间明显高于单伤组 ;2伤口愈合过程中 Bax、Bcl 2表达随着细胞凋亡的增加呈规律性改变 :Bax表达明显增强 ,且与细胞凋亡发生有良好的相应关系 ;而 Bcl 2呈明显下降趋势。结论 :Bax和 BclObjective:To study the relationship between the expressions of Bax and Bcl2 genes and apoptosis and delayed wound healing in simple and radiation combined wound in rats.Methods:Apoptosis was demonstrated by TdTmediated dUTP nick end labeling (TUNEL) technic and expression of Bax and Bcl2 mRNA was determined by in situ hybridization.Results:The frequency and lasting time of apoptosis in radiation combined wound group were significantly higher than those in simple wound group.In the process of wound healing,the expression of Bax and Bcl2 genes showed regular changes:Bax expression increased markedly with the increment of apoptosis and showed a high correlation with the apoptosis,while the expression of Bcl2 decreased obviously.Conclusions:Bax and Bcl2 genes may play an important role in the regulation of apoptosis and delayed wound healing in radiation combined wound in rats.

关 键 词：创伤 放射复合伤口 BAX基因 BCL-2基因 细胞凋亡 愈合延迟 

分 类 号：R818[医药卫生—放射医学]