疏水色谱法及其在生物大分子分离纯化中的应用  

Hydrophobic interaction chromatography and its application in purification of biopolymers

作　　者：王云[1] 常玲[2] 吴金男[1] 

机构地区：[1]常熟高等专科学校生化系 [2]常熟高等专科学校人事处

出　　处：《烟台师范学院学报（自然科学版）》2002年第2期135-140,共6页Yantai Teachers University journal(Natural Science Edition)

摘　　要：综合评述了疏水相互作用色谱法 ( hydrophobic interaction chromatography,HIC)The development of hydrophobic interaction chromatography (HIC) is mainly concerned in this article.Several theories for the retention mechanism of protein in HIC and main parameters related to chromatographic behavior are reviewed respectively.The selected examples of proteins purified by HIC are also presented.

关 键 词：疏水相互作用色谱法 分离纯化 生物大分子 疏水色谱填料 蛋白质 折叠机理 生物工程 

分 类 号：Q510.3[生物学—生物化学]