儿童幽门螺杆菌感染途径的研究  

Exploration for possible source of Helicobacter pylori infection in children

作　　者：姜海行[1] 梁淡湄[1] 王琳琳[1] 陈振侬[1] 单庆文[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021

出　　处：《临床儿科杂志》2002年第6期329-331,T001,共4页Journal of Clinical Pediatrics

基　　金：广西壮族自治区科技厅基金资助 (基金编号 :9811040)

摘　　要：为探讨儿童幽门螺杆菌感染的可能途径,对198例儿童及69例一级亲属 ,采用PCR方法扩增唾液和胃粘膜H.pylori尿素酶C(UreC)基因(820bp) ,扩增产物分别用限制性核酸内切酶HhaI与AluI酶切消化 ,得出H.pylori的酶切类型(RFLPc)。结果显示 ,H.pyloriUreC基因扩增片断经限制性核酸内切酶HhaI与AluI酶切后 ,分别得到9种和7种不同的酶切图谱 ,结合两种酶切类型共确定22个独立的酶切类型 ;在31个儿童与亲属同时感染的家庭中 ,25.0 %(7/28例)的儿童唾液中可扩增出H.pylori,且唾液与相应胃粘膜中的幽门螺杆菌RFLPc酶切类型一致 ;83.9%(26/31例)儿童感染的H.pylori与其亲属感染的H.pylori类型相同 ,16.1%(5/31例)不相同(P<0.05)。表明PCR_RFLP分析H.pyloriUreC基因的酶切类型分辨率较高 ,直接扩增样本H.pylori方法简便 ,可用于H.pylori的流行病学研究 ;唾液可作为家庭内成员H.pylori感染的传播因素。To explore the possible source of Helicobacter pylori (H.pylori) infection in children,UreC gene in both of saliva and gastric mucosa collected by gastroscopy were amplified by PCR_RFLP method in 196 children with upper digestive tract symptoms and 69 first_degree relatives among these children.Amplified produces were further digested by restrietion endonucleases Hha I and Alu I for obtaining RFLPc patterns of H.pylori.The results showed that nine digest patterns by Hha I and seven digest patterns by Alu I were obtained in amplified produces of UreC gene.A total of 22 RFLRc patterns of H.pylori were confirmed based on the digest patterns with combination of restriction endonucleases Hha I and Alu I.It was found that H.pylori in saliva could be amplified in 25%(7/28)of children among 31 infectious families,and RFLPc patterns were identical in both of saliva and gastric mucosa.Identical digest patterns were also confirmed in 83.9%(26/31)of infectious families(P<0.05).It is concluded that PCR_RFLP method is a sensitive and reliable way for the investigation of epidemiology of H.pylori infection.Accordant RFLPc patterns were confirmed in both of saliva and gastric mucosa and infectious children and their first_degree relatives,that suggest oral_oral transmission be a main media type to infect H.pylori in children probably.

关 键 词：儿童 幽门螺杆菌 传播 聚合酶链反应-限制性片段长度多态性 慢性胃炎 PCR-RFLP技术 

分 类 号：R725.7[医药卫生—儿科]