箭根薯的试管繁殖  

In vitro propagation of Tacca chantrieri

作　　者：何惠英[1] 兰芹英[1] 张艳军[1] 

机构地区：[1]中国科学院西双版纳热带植物园,云南勐腊666303

出　　处：《广西农业生物科学》2002年第2期108-110,共3页Journal of Guangxi Agricultural and Biological Science

基　　金：中国科学院生物分类区系学科发展特别支持项目 (980 3 0 2 )

摘　　要：本实验用箭根薯成熟种子为材料 ,培育出无菌幼苗 ,再用幼叶、叶柄进行试管繁殖。经实验得出各阶段适宜的培养基分别为 :(1 )种子萌发 :MS+6BA1 mg/L (单位下同 ) +NAA0 .1 ;(2 )诱导愈伤组织 :MS+6BA1 +2 ,4D1 .5 +KT0 .2 ;(3 )丛芽诱导 :MS+6BA0 .5 +NAA0 .5 ;(4 )生根培养 :1 /2 MS+NAA0 .5。Tacca chantrieri bear many seeds,but only few of the seeds (about 12%) can germinate,so tissue culture would be a good means for multiplying plants of T.chantrieri. In this experiment,young leaves and leaf stalks,obtained from mature T.chantrieri seeds,were used as explants for in vitro propagation.The appropriate media for respective culture stages were as follows.The medium of seed germinarion was MS+6 BA 1mg/L+NAA0 1mg/L,that of callus inducing was MS+6BA1mg/L+2,4D1 5mg/L+KT0 2mg/L,that of shoot inducing was MS+ 6BA 0 5mg/L+NAA 0 5 mg/L,that of rooting was 1/2 MS+NAA 0 5 mg/L.

关 键 词：箭根薯 愈伤组织 组织培养 生根培养 移栽 

分 类 号：S567.23[农业科学—中草药栽培]