氯通道阻断剂对KCl、5-HT引起的兔脑椎基底动脉血管环收缩效应的影响  

Effects of chloride channel blockers on KCl and 5-HT-induced contractile response at rabbit basilar artery rings

作　　者：丁岩[1] 关永源[1] 熊大志[1] 贺华[1] 

机构地区：[1]中山大学医学院药理学教研室,广州510089

出　　处：《中国药理学通报》2002年第3期267-271,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题 No 3 9970 849;国家科技部攀登计划资助课题 No国科基字 [1999] 0 45号;2001年广东省自然科学基金团队项目资助课题

摘　　要：目的　探讨不同种类氯通道阻断剂在不同激动剂引起的脑血管平滑肌收缩反应中的作用。方法　记录离体兔脑椎基底动脉环收缩反应。结果　①氯通道阻断剂DIDS、Furosemide及NPPB均浓度依赖性抑制高K+ 及 5 HT引起血管环收缩反应 ,DIDS、Furosemide及NPPB对 5 HT引起收缩的抑制作用明显强于对KCl的作用 ;②在 5 HT引起血管收缩反应中 ,给予SK&F96 36 5引起血管最大舒张的基础上 ,DIDS、Furosemide及NPPB均可引起血管进一步舒张。结论　氯通道可能参与了由高K+ 去极化和 5AIM To study the effects of different kinds of chloride channel blockers on KCl and 5 HT induced contractile responses in cerebrovascular smooth muscle. METHOD The tension of rabbit basilar artery rings was measured. RESULTS ① DIDS, furosemide and NPPB inhibited the responses to KCl and 5 HT in a concentration dependent manner. The effects of DIDS, furosemide and NPPB on the response to 5 HT were stronger than that to KCl. ②When SK&F 96365 evoked a maximum inhibitory effect on 5 HT induced response, subsequent additions of DIDS, furosemide and NPPB could further produced vascular relaxation. CONCLUSION The chloride channel participates KCl and 5 HT induced contractile responses in cerebral basilar artery.

关 键 词：兔 脑 椎基底动脉血管环收缩效应 CA^2+通道 Cl^-通道阻断剂 KCL 5-HT 

分 类 号：R322.121[医药卫生—人体解剖和组织胚胎学] R331.33[医药卫生—基础医学]