增生性瘢痕组织中转化生长因子-β异构体与受体含量变化及对瘢痕形成的影响  

CHARACTERISTICS AND EFFECT OF THREE TRANSFORMING GROWTH FACTOR -β ISOFORMS AND THEIR RECEPTOR(I) ON SCAR FORMATION

作　　者：陈伟[1] 付小兵[1] 孙同柱[1] 孙晓庆[1] 盛志勇[1] 

机构地区：[1]解放军第304医院,北京100037

出　　处：《中国修复重建外科杂志》2002年第4期252-255,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重点基础研究发展规划资助项目 (G1 9990 542 0 4 );国家杰出青年科学基金资助项目 (3952 50 2 4 ) ;国家自然科学基金资助项目 (39870 731 )

摘　　要：目的　观察转化生长因子 - β三种异构体 (TGF- β1 、β2 和 β3)和其受体 - I[TGF- βR(I) ]在增生性瘢痕和正常皮肤组织中的表达特征及其对瘢痕形成的影响。方法　 16块被测标本中包括增生性瘢痕 8例 (块 ) ,其对应的正常皮肤组织 8块。用免疫组织化学方法和常规病理技术分析其在增生性瘢痕和正常皮肤组织中的定位和表达量的变化规律。结果　正常皮肤组织中 ,TGF-β1 、β2 和β3细胞因子的阳性信号主要见于表皮细胞、汗腺及毛囊细胞的胞浆和细胞外基质中 ,TGF-βR(I)蛋白则主要定位于表皮细胞和部分成纤维细胞的细胞膜上。增生性瘢痕组织中 ,TGF-β1 、β3细胞因子的阳性信号主要见于表皮基底层细胞内 ,TGF- β2 的阳性颗粒则分布于表皮细胞和部分成纤维细胞的胞浆和胞核内。与正常皮肤组织相比 ,增生性瘢痕组织内 TGF- β1 和 β3含量明显下降 ,TGF- β2 含量变化不显著 ,而 TGF- βR(I)阳性颗粒含量和分布无明显改变。结论　 TGF- β1 、β2 和 β3在增生性瘢痕组织中含量和分布的不同变化规律显示 ,这三种细胞因子可能参与增生性瘢痕的形成 ,而 TGF-βR(I)蛋白及其下游的信号分子是否与瘢痕的形成相关 ,还需深入地探讨。Objective To observe the differences in protein contents of three transforming growth factor beta(TGF β) isoforms, β 1, β 2, β 3 and their receptor(I) in hypertrophic scar and normal skin and to explore their influence on scar formation. Methods Eight cases of hypertrophic scar and their corresponding normal skin were detected to compare the expression and distribution of TGF β 1, β 2, β 3 and receptor(I) with immunohistochemistry and common pathological methods. Results Positive signals of TGF β 1, β 2, and β 3 could all be detected in normal skin, mainly in the cytoplasm and extracellular matrix of epidermal cells; in addition, those factors could also be found in interfollicular keratinocytes and sweat gland cells; and the positive particles of TGF β R(I) were mostly located in the membrane of keratinocytes and some fibroblasts. In hypertrophic scar, TGF β 1 and β 3 could be detected in epidermal basal cells; TGF β 2 chiefly distributed in epidermal cells and some fibroblast cells; the protein contents of TGF β 1 and β 3 were significantly lower than that of normal skin, while the change of TGF β 2 content was undistinguished when compared with normal skin. In two kinds of tissues, the distribution and the content of TGF β R(I) had no obviously difference. Conclusion The different expression and distribution of TGF β 1, β 2 andβ 3 between hypertrophic scar and normal skin may be associated with the mechanism controlling scar formation, in which the role of the TGF βR (I) and downstream signal factors need to be further studied.

关 键 词：转化生长因子-β异构体 转化生长因子-β受体-Ⅰ 增生性瘢痕 皮肤 

分 类 号：R622[医药卫生—整形外科]