骨髓基质干细胞作软骨组织工程种子细胞研究  

POTENTIAL SEEDING CELLS FOR CARTILAGE TISSUE ENGINEERING——BONE MARROW STROMAL STEM CELLS

作　　者：孔清泉[1] 项舟[1] 杨志明[1] 

机构地区：[1]四川大学华西医院骨科,成都610041

出　　处：《中国修复重建外科杂志》2002年第4期277-280,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家重点基础研究发展规划项目 (973) :组织工程化软骨组织的构建 (G1 9990 5430 8) ;国家自然科学基金重点资助项目(39830 1 0 0 )

摘　　要：目的　综述近年来骨髓基质干细胞体外培养、定向分化为软骨细胞的条件及相关研究进展。方法　广泛查阅相关文献 ,对上述研究进展进行整理、综合和分析。结果　骨髓基质干细胞易于分离培养 ,体外增殖能力强 ,传代多次后仍保持有多分化潜能 ;体内成软骨能力明确 ;但骨髓基质干细胞定向分化的软骨细胞 ,不是终末分化阶段 ,而只是一个中间阶段。结论　骨髓基质干细胞以其自身多方面的特点已成为软骨组织工程的另一种可选择的种子细胞 ,但其进一步分化为成熟软骨细胞的条件尚需进一步研究。Objective To review the recent research progress of bone marrow stromal stem cells (BMSCs) in the conditions of culture in vitro, chondrogenic differentiation, and the application in cartilage tissue engineering. Methods Recent original articles related to such aspects of BMSCs were reviewed extensively. Results BMSCs are easy to be isolated and cultivated. In the process of chondrogenesis of BMSCs, the special factors and interaction between cells are investigated extensively. BMSCs have been identified to form cartilage in vivo. One theory is the committed chondrocyte from BMSCs is only a transient stage. Conclusion BMSCs are the alternative seeding cells for cartilage tissue engineering. The conditions promoting mature chondrocyte should be further investigated.

关 键 词：组织工程 骨髓基质干细胞 软骨 体外培养 

分 类 号：R687.34-33[医药卫生—骨科学]