苦参总碱注射液的药动学的研究  

Study on pharmacokinetics of sophora flavescens injection in rabbits

作　　者：陈亚飞[1] 田颂九[1] 宋景政[1] 孙曾培[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国药学杂志》2002年第7期524-526,共3页Chinese Pharmaceutical Journal

摘　　要：目的　建立了兔血浆中苦参类生物碱的毛细管电泳分离测定法。方法　在血浆中加入内标西咪替丁后 ,用甲醇进行蛋白沉淀 ,高速离心后 ,直接进样测定。运行缓冲液为 0 .2mol·L-1Tris 4 0mmol·L-1磷酸二氢钠溶液 2 0 %异丙醇 ,用磷酸调pH至 5 .5 ,检测波长为 2 0 5nm。 结果　本测定方法的提取回率为 94 .6 4 %～ 98.78% ,对血浆中氧化槐果碱的最低检测浓度为 0 .2 μg·L-1,氧化苦参碱的最低检测浓度为 1.0 μg·L-1,氧化槐果碱和氧化苦参碱的线性范围分别为 0 .4 1～ 72 .5 μg·L-1和 1.95～ 2 6 8.5 μg·L-1,相关系数分别为 0 .9992和 0 .9994。兔静注 10 0mg·kg-1苦参总碱注射液后 ,用本法测定了兔血浆中氧化槐果碱和氧化苦参碱的浓度经时变化过程 ,并对其药动学参数进行估算。结论　用本法同时测定兔血浆中的氧化槐果碱和氧化苦参碱 ,结果满意 。OBJECTIVE: To establish a capillary electrophoresis method for determing the concentrations of oxysophocarpine and oxymatrine in rabbit plasma. METHODS: The internal standard was cimetidine. The protein in plasma was precipitated with methanol and centrifuged at high speed. The supernatant was directly injected and assayed by CE method. The running buffer was 0.2 mol&middotL-1 Tris-40 mmol&middotL-1 sodium phosphate monobasic-20 % isopropanol, adjusted pH to 5.5 with phosphoric acid. The wave-length of detection was 205 nm. RESULTS: The recovery of this method was in the range of 94.64%∼98.7%. The detection limits of oxysophocarpine(OSC) and oxymatrine(OMT) were 0.2 and 1.0 μg&middotL-1 respectively. The standard curves was linear over the range of 0.41∼72.5 μg&middotL-1 for OSC and 1.95∼268.0 μg&middotL-1 for OMT respectively. The correlation coefficients of OSC and OMT were 0. 9992 and 0. 9994. The concentrations of OSC and OMT at different time were analyzed and estimated for their pharmacokinetic parameters. CONCLUSION: The results were satisfactory and conformed to open two-compartment model.

关 键 词：苦参总碱注射液 氧化槐果碱 氧化苦参碱 高效毛细管电泳法 药动学 

分 类 号：R969.1[医药卫生—药理学] R979.1[医药卫生—药学]