猪瘟野毒混合毒实验室感染的研究  

Experimental Infection of the Mixed Field Isolated Strains of Hog Cholera Virus

作　　者：赵耘[1] 王在时[1] 王琴[1] 李博[1] 丘惠深[1] 宁宜宝[1] 宋立[1] 张广川[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国预防兽医学报》2002年第4期273-276,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家重点基础研究发展规划项目 (G19990 1190 4 )

摘　　要：本研究以本所近年来所分离的高、中、低三株不同毒力 (HeNXH3.98、JL1 94和FJFQ1 99)的猪瘟野毒混合毒对敏感猪进行实验室感染试验 ,利用HCFA、RT_PCR及序列测定进行检测和分析 ,结果 2头试验猪均在感染后 2周发病死亡 ,表现典型的猪瘟临床症状 ;序列测定及分析结果表明感染后 1周及 2周 2头实验感染猪所分离的病毒E2基因主要抗原编码区序列完全一致 ,核苷酸及氨基酸同源性均为 10 0 % ;与感染毒株序列比较 ,2头试验感染猪所分离的病毒E2基因主要抗原编码区序列与JL1 94株序列完全一致 ,核苷酸及氨基酸同源性均为 10 0 % ,且基因分群在同一群 ;而与HeNXH3.98和FJFQ1 99株序列则有一定的差异 ,且不在同一基因群或基因亚群。说明在多个猪瘟病毒存在的情况下 ,敏感猪存在对猪瘟病毒的优势选择。本试验的条件下 3株不同毒力的猪瘟野毒混合感染以中等毒力毒株JL1Experimental infection on sensitive pigs was done with 3 mixed field isolated strains of Hog Cholera Virus(HeNXH3.98,JL 1.94 and FIFQ1.99),its toxicity was different and isolated by our institute in recent years.The method of analysis and examine were HCFA,RT_PCR and sequencing.The 2 infected pigs showed typical clinical symptoms of Hog Cholera and dead after infected 2 weeks.Analysis of sequence showed that the major antigen coding region sequences of E2 gene of isolated strains from 2 infected pigs were no difference after infected 1 and 2 weeks.The identity of nucleotide sequence and amino acid sequences was 100%.Compared with the infected strains,the major antigen coding region sequences of E2 gene of isolated strains from 2 infected pigs were identity with JL 1.94 strain.The identity of nucleotide sequence and amino acid sequences was 100%,and all belonged to the same gene group.But the major antigen coding region sequences of E2 gene of isolated strains from 2 infected pigs had little difference with the sequence of HeNXH3.98 and FJFQ1.99 strains,and they were not in the same gene group or gene subgroup.The results of this study illustrated that sensitive pigs have superior selection if there are many hog cholera viruses.In this experimental condition,the superior strain of infection of 3 different toxicity hog cholera field isolated strains was middle toxictiy strain of hog cholera field isolated strain.

关 键 词：猪瘟野毒混合毒 感染 序列分析 优势选择 

分 类 号：S852.65[农业科学—基础兽医学]