苏丹草品种纯度鉴定技术的研究——过氧化物酶同功酶标记  

Study on technology of variety identification in sudangrass --POD isozyme marking

作　　者：张建全[1] 王彦荣[1] 

机构地区：[1]兰州大学草地农业科技学院,甘肃草原生态研究所兰州730020

出　　处：《草业学报》2002年第2期95-99,共5页Acta Prataculturae Sinica

基　　金：国家重点基础研究发展规划"中国北方沙漠化过程及防治"(G2 0 0 0 0 4 870 4 );国家自然科学基金项目 (30 1 70 672 );农业部"九五"畜牧业重点项目专题 (95牧 - 0 2 - 0 6- 0 6)资助

摘　　要：对我国审定登记的 5个苏丹草品种 ,应用幼苗叶片过氧化物酶同功酶电泳技术进行品种鉴定。结果表明 ,5个品种过氧化物酶同功酶表现出明显的谱带差异 :首先根据相对迁移率 (Rf)为 0 .834位置酶带的形状将 5个品种划分为 3组。其中 ,新苏 2号和盐池两品种呈连续的条形 ,宁农和乌拉特 1号下方有 1三角形谱带 ,而奇台苏丹草在该谱带左下方有 1圆点。其次 ,新苏 2号和盐池两品种在 Rf为 0 .4 5 5和 0 .4 93位置存在酶带 ,但新苏 2号酶带颜色明显深于盐池。另外 ,宁农和乌拉特 1号相比 ,Rf为 0 .834位置下方的三角形谱带乌拉特 1号居中而宁农偏右 ,可据此进一步区分该 2品种。Suitability of seedling POD isozyme PAGE was studied for identifiing 5 varieties of sudangrass (Sorghum sudanense) registered in China. Result showed that patterns of POD isozyme PAGE were markedly different among the varieties. Three groups were, firstly classified according to shape of band at of Rf 0.834. Among them, Xinsu No.2 and Yanchi belong to one group being with continuous stripe band; Qitai has a distinguish band of dot shape at left down side; and the other two varieties have a distinguish triangle band below Rf 0.834. Secondly, there are two bands are in common at Rf 0.455 and 0.493 between the varieties of Xinsu No.2 and Yanchi, however, the band of Xinsu No.2 is more darker than Yanchi. In addition, between the varieties of Ningnong and Wulate No.1, the triangle band at Rf 0.834 for Ningnong is located at right side, while that for Wulate No.1 is in the middle. This study suggested that the seedling POD isozyme PAGE could be as an efficient technique for variety identification in sudangrass.

关 键 词：苏丹草 品种鉴定 过氧化物酶同功酶 聚丙烯酰胺电泳 

分 类 号：S544[农业科学—作物学]