人参茎叶皂苷与小剂量雌激素联合用药防治去卵巢大鼠骨丢失  

COMBINATION OF GINSENOSIDES WITH LOW DOSE ESTROGEN SHOWED SYNERGETIC EFFECT ON OVARIECTOMY INDUCED OSTEOPENIA IN RATS

作　　者：崔燎[1] 吴铁[1] 刘晓青[1] 刘玉瑜[1] 李青南[2] 

机构地区：[1]广东医学院药理教研室,广东湛江524023 [2]广东医学院骨生物研究室,广东湛江524023

出　　处：《药学学报》2002年第7期501-505,共5页Acta Pharmaceutica Sinica

基　　金：广东医学院留学回国人员启动基金 ( 990 9-XJ990 4)

摘　　要：目的　研究人参茎叶皂苷 (GS)和 17α 炔雌醇 (EE)对去卵巢大鼠骨质疏松的作用。方法　大鼠双侧卵巢去除术后预防用药 10周。用骨组织形态计量学方法 ,测定大鼠胫骨近端松质骨静态参数和动态参数 ,并观察血清生化指标和器官指数。结果　EE 30 0 μg·kg- 1 ·d- 1 可完全对抗去卵巢大鼠体重增加、血清总胆固醇增加、骨转换率增高和骨量丢失。EE 10 0 μg·kg- 1 ·d- 1 对骨量丢失有部分对抗作用。GS 30 0 μg·kg- 1 ·d- 1 对骨量的效应与EE 10 0 μg·kg- 1·d- 1 效应相似。低剂量GS和EE联合用药效应超过两者单独应用。结论　GS和EE低剂量联合用药对去卵巢大鼠骨丢失的骨量增加有协同作用。AIM To determine whether low dose of estrogen in combination with ginsenosides can completely prevent bone loss in ovariectomized rats. METHODS Four-month-old ovariectomized rats were treated either with 100 and 300 mg·kg -1 of ginsenosides or 30 and 100 μg·kg -1 of 17α-ethynylestradiol alone, or ginsenosides 100 mg·kg -1 in combination with 17α-ethynylestradiol 30 μg·kg -1 for 10 weeks. Double in vivo fluorochrome labeling was made. The undecalcified longitudinal proximal tibial metaphyseal sections were processed and stained with Goldner's trichrome for histomorphometric analysis of the bone. RESULTS Body weights and serum cholesterol were increased in ovariectomized (OVX) rats. The rats lost 74% of bone volume and high bone turnover was induced after OVX compared with the sham group. Bone volume increased by 205% in the high dose estrogen treated group while it was increased by 105% in the low dose group. The two doses of estrogen were shown to inhibit osteoclasts surface (by -65% and -55% , P<0.01) and decrease bone turnover rate (by -85% and -83% , P<0.01). High dose of estrogen was found to inhibit growth and stimulate uterin weight gain in rats while low dose did not. High dose of ginsenosides increased bone volume by 84% (P<0.01) and decreased bone turnover rate by -64% (P<0.05) while lower dose of ginsenosides did not. However, low dose ginsenosides combinated with low dose estrogen achieved well preventive effects: increase of 202% in bone volume, decrease of 66% in bone turnover rate and 72% in osteoclasts surface. The combined effect in preventing bone loss equals to that the high dose of estrogen alone did. CONCLUSION Use of low dose of estrogen plus ginsenosides showed synergistic effect on prevention of osteoporosis induced by ovariectomy.

关 键 词：雌激素 人参茎叶皂苷 17α-炔雌醇 中西药结合 联合用药 骨质疏松 骨组织形态计量学 绝经 

分 类 号：R681[医药卫生—骨科学]