人Jurkat细胞GAGA样元件结合蛋白cDNA的克隆  

Cloning cDNA Encoding For GAGA-like Element Binding Proteins in Human J urkat Cells

作　　者：莫志成[1] 陆瑜[1] 高超[1] 吴宁华[1] 沈珝琲[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《中国医学科学院学报》2002年第3期269-271,共3页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(39930050)资助

摘　　要：目的探讨人Jurkat细胞中是否存在与GAGA样元件相互作用的结合蛋白。方法采用酵母单杂交体系,在HTLV-1转化的Jurkat细胞cDNA文库中筛选与GAGA样元件相互作用的蛋白;提取人Jurkat细胞总RNA,应用反转录反应标记cDNA探针,与候选的阳性克隆杂交。结果共获得9个阳性克隆,其中6个阳性克隆的插入片段可以与cDNA探针杂交。结论在人Jurkat细胞中有与GAGA样元件相关的结合蛋白。Objective To explore GAGA-like element binding protein in human cells.Methods Yeast one-hybrid system was used to screen the GAGA-like element binding proteins in HTLV-1transformed Jurkat cell cDNA fusion library.Total RNA extracted from Jurkat cells was first labeled by reverse transcription,and was taken as cDNA probe to hybridize with the candidate positive clones.Results9positive clones were obtained,and6out of the9clones were positively hybridized with the cDNA probe.Conclusions 6candidate clones encoding for GAGA-like element binding proteins were obtained from Jurkat cells for further investigation.

关 键 词：JURKAT细胞 酵母单杂交体系 GAGA元件 基因调控 

分 类 号：Q343.1[生物学—遗传学]