Fas,Fas-L和Caspase-3在增生性瘢痕中表达特征及其对瘢痕形成的影响  

CHARACTERISTICS OF Fas, Fas-L AND Caspase-3 EXPRESSION IN HYPERTROPHIC SCARS AND THEIR EFFECTS ON SCAR FORMATION

作　　者：陈伟[1] 付小兵[1] 孙同柱[1] 赵志力[1] 王振辉[1] 盛志勇[1] 

机构地区：[1]解放军第304医院

出　　处：《解放军医学杂志》2002年第7期580-581,I001,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家重大基础研究规划资助项目 (编号G1 9990 542 0 4 );国家杰出青年科学基金 (编号 3952 50 2 4 );国家自然科学基金 (编号 30 1 70 996)

摘　　要：探讨Fas、Fas L和Caspase 3在增生性瘢痕和正常皮肤组织内的表达特征及其对瘢痕形成的影响。用免疫组化方法和常规病理技术检测这三种蛋白在 8例增生性瘢痕和 8例正常皮肤中的表达变化规律。结果发现 ,在正常皮肤中 ,Fas和Fas L主要分布于表皮细胞、部分成纤维细胞的胞浆和胞膜内 ,Caspase 3则定位于这些细胞的胞浆中。Fas和Fas L在增生性瘢痕的表皮基底层细胞内有少量分布 ,阳性细胞率显著降低(P <0 0 5 ) ;Caspase 3的阳性细胞率也明显降低 (P <0 0 5 ) ,在表皮角质形成细胞的胞浆内有轻微表达。提示增生性瘢痕的发生可能与Fas、Fas L和Caspase 3蛋白表达降低、由这三种蛋白介导的细胞凋亡受阻相关。To investigate the different expression of Fas, Fas L and Caspase 3 in hypertrophic scars and normal skins in order to explore their influences on scar formation, the expression intensity and distribution of Fas, Fas L and Caspase 3 were detected by immunohistochemistry and pathological methods in 8 cases of hypertrophic scars and 8 cases of normal skins. In normal skins, Fas and Fas L were mainly located in the cellular membranes and cytoplasms of epidermal cells and some fibroblasts. Caspase 3 was mostly distributed in epidermal basal cells and some fibroblasts. In hypertrophic scars, Fas and Fas L were principally existed in the membranes and cytoplasms of epidermal keratinocytes. The positive cellular ratio of two proteins was significantly reduced ( P <0 05). The cells containing Caspase 3 protein were chiefly the basal lamina cells of epidermis. The positive cellular ratio of the protein was also notably decreased in comparison with that of normal skins ( P <0 05). These results suggest that the formation of hypertrophic scars may be associated with the reduction of Fas, Fas L and Caspase 3 protein expression, which leads to the inhibition of apoptosis.

关 键 词：增生性瘢痕 瘢痕 免疫组织化学 CD95抗原 FAS基因 Fas-L基因 CASPASE-3基因 发病机制 基因表达 

分 类 号：R619.6[医药卫生—外科学]