TNF对离体孵育骨骼肌的蛋白代谢与泛素系统基因表达的影响  

THE EFFECTS OF TNF-α ON THE PROTEIN METABOLISM AND THE UBIQUITIN SYSTEM GENE EXPRESSION IN ISOLATED SKELETAL MUSCLES

作　　者：申传安[1] 柴家科[1] 盛志勇[1] 

机构地区：[1]解放军第304医院

出　　处：《解放军医学杂志》2002年第7期582-584,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金 (编号 39970 71 6);全军医学科研"十五"计划重大项目 (编号 0 1Z0 95)

摘　　要：为研究TNF α对离体孵育骨骼肌蛋白代谢及泛素系统基因表达的影响 ,采用含重组大鼠TNF α(6 0 0 0U/ml)的孵育液有氧孵育大鼠伸趾长肌 (EDL) ,对照组孵育液除不含TNF α外其余成分与实验组相同 ,高效液相色谱法测定总蛋白和肌纤维蛋白的代谢率 ,核糖核酸印迹法检测EDL泛素mRNA和C2 mRNA的表达变化。结果显示 ,实验组大鼠EDL总蛋白和肌纤维蛋白分解代谢率与对照组相比差异无统计学意义 ,但EDL泛素mRNA(2 4kb)和C2 亚基mRNA的表达较对照组分别升高约 15 1%和 5 6 % ,差异有统计学意义。提示TNF α能与骨骼肌细胞直接发生作用 ,增强细胞内泛素蛋白酶体途径的活性 ,但TNF α能否直接增强骨骼肌蛋白分解代谢尚有待进一步探讨。To study the effects of TNF α on the protein metabolism and the ubiquitin system gene expression in isolated skeletal muscles, after dissecting and isolating the extensor digitorium longus (EDL) muscles, the in vitro oxygen rich muscle incubation system and as high performance liquid chromatography were used to assess proteolytic rate of the samples. The EDL muscles in study group were incubated with media containing 6 000 U/ml recombinant rat TNF α. In control group, the media were of the same composition as that of the study group except recombinant rat TNF α. The expressions of ubiquitin mRNA and C 2 mRNA in rat EDL muscles were determined by Northern blot analysis. No notable difference was observed in the total and myofibrillar proteolytic rate in EDL muscles between the two groups. The expressions of ubiquitin mRNA(2 4kb) and C2 mRNA of EDL muscle incubated with medium containing TNF α were increased by 151% and 56%, respectively, as compared with those in control group. TNF α could directly strengthen the function of ubiquitin dependent proteolytic system, but further studies are necessay to elucidate whether TNF α could directly increase the proteolytic rate in skeletal muscle.

关 键 词：基因表达 蛋白降解 骨骼肌 肿瘤坏死因子 泛素 RNA印迹法 高效液相色谱法 脓毒症 负氮平衡 

分 类 号：R631[医药卫生—外科学]