蔗糖对水稻幼苗叶片谷氨酰胺合成酶和1,5-二磷酸核酮糖羧化酶/加氧酶的影响  

Effect of Sucrose on Glutamine Synthetase and Ribulose-1,5-bisphos- phate Carboxylase/Oxygenase in Leaves of Rice Seedlings

作　　者：袁永泽[1] 林清华[1] 张楚富[1] 王其海[1] 魏国威[1] 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2002年第3期219-222,共4页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助 (编号 :3 9870 5 2 7

摘　　要：报道了在光照和暗处培养下 ,不同浓度的蔗糖对水稻幼苗叶片 GS及其同工酶、1,5 -二磷酸核酮糖羧化酶/加氧酶 (Rubisco)的影响。无论是在光照或在暗处 ,蔗糖对 GS活性均有抑制作用 ,尤其是在较高蔗糖浓度下作用更为明显 ;虽然 Rubisco及可溶性蛋白的水平在光照和暗处有显著的差别 ,但蔗糖对其未见明显影响。 Native-PAGE与活性染色表明 ,在光照下或在暗处 ,蔗糖对 GS2的抑制作用随蔗糖浓度升高而加强 ,但对 GS1未有明显影响。这些结果提示 ,在水稻幼苗生长中 ,蔗糖不能象光一样诱导叶片In the current report, we have investigated that the effect of sucrose on GS and its isozymes as well as ribulose 1,5 bisphosphate carboxylase/oxygenase(Rubisco) of the leaves of rice seedlings. GS activity was decreased with the increase of sucrose concentrations whether the illumination of light or the dark. The levels of the soluble proteins and Rubisco were not obviously influenced by sucrose at the illumination of light or the dark although their changes were diffe rent . Native PAGE and activity staining showed that the inhibition of sucrose to GS2 expression was strengthened with sucrose concentrations but GS1 was not clearly affected in the illumination of ligh or the dark. These results suggested that sucrose can not induce the expression of GS in leaves of rice seedlings as light.

关 键 词：蔗糖 水稻幼苗 叶片 谷氨酰胺合成酶 1 5-二磷酸核酮糖羧化酶 加氧酶 影响 

分 类 号：Q946.5[生物学—植物学] S511.01[农业科学—作物学]