大凉疣螈脑源性神经营养因子(BDNF)基因的克隆与序列分析  

CLONING AND SEQUENCE ANALYSIS OF BRAIN-DERIVED NEUROTROPHIC FACTOR GENE OF TYLOTOTRITON TALIANGENSIS

作　　者：曹玫[1] 杨玉华[1] 张义正[1] 

机构地区：[1]四川大学生命科学学院分子生物学实验室,成都610064

出　　处：《应用与环境生物学报》2002年第3期314-316,共3页Chinese Journal of Applied and Environmental Biology

摘　　要：The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,RefThe full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,Ref 9

关 键 词：大凉疣螈 脑源性神经营养因子 BDNF 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]