家蚕微粒子病原虫(Nosema bombycis)小亚基核糖体RNA全基因的克隆及其二级结构的构建  

Analyses of small subunit ribosomal RNA sequence of the microsporidium,Nosema bombycis and its secondary structure

作　　者：王见杨[1] 黄可威[1] 陆长德[2] 

机构地区：[1]中国农业科学院蚕业研究所,镇江212018 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《昆虫学报》2002年第3期290-295,共6页Acta Entomologica Sinica

基　　金：国家"九五"科技攻关专题 ( 96 6 16 0 2 0 3);江苏省"九五"科技攻关课题 (BE96 36 9 1)

摘　　要：在用PCR技术扩增、克隆、测序了家蚕微粒子病原虫Nosemabombycis (镇江株 )小亚基核糖体RNA基因核心序列(5′ 端起 12 0 0bp)的基础上 ,用SSP PCR技术克隆了核心序列 3′ 端下游序列 ,从而获得了家蚕微粒子病原虫小亚基核糖体RNA基因的全序列共 12 33bp。用RnaViz、Forcon、DCSE等生物软件构建了家蚕微粒子病原虫小亚基核糖体RNA的二级结构 ,与其它微孢子虫及真核生物小亚基核糖体RNA的二级结构相比 ,该二级结构缺乏螺旋 10、E10 1、 11、 18、E2 3 n和43。The nucleotide sequence (1 205 bp) of small subunit ribosomal RNA(SSUrRNA) of the microsporidium, Nosema bombycis (Zhenjiang), was amplified by polymerase chain reaction(PCR). Its 3′-end was obtained using the single specific primer-PCR technique with the primers matching the highly conserved parts of SSUrRNA genes cloned. The entire coding region for SSUrRNA of N. bombycis (Zhenjiang) was 1 233 bp as described elsewhere. The sequence shared high homology with those of many microsporidia, especially N. apis. The secondary structure of the SSUrRNA was constructed with RnaViz, Forcon and DCSE. There were no helices such as 10, E10-1, 11,18, E23-n and 43 in it.

关 键 词：家蚕微粒子病原虫 SSP-PCR 小亚基核糖体RNA SSUrRNA 全基因 二级结构 螺旋 

分 类 号：Q965.8[生物学—昆虫学] Q522