Salvia miltiorrhiza monomer IH764-3 induces hepatic stellate cell apoptosis via caspase-3 activation  

作　　者：ZhanXL LiuL 等 

机构地区：[1]DepartmentofGastroenterology,TheSecondHospitalofHebeiMedicalUniversity,Shijiazhuang05000,HebeiProvince,China [2]DepartmentofGastroenterology,TheSecondHospitalofHebeiMedicalUniversity,Shijiazhuang05000,He

出　　处：《World Journal of Gastroenterology》2002年第3期515-519,共5页世界胃肠病学杂志（英文版）

基　　金：Fund of the Scientific and Technical Department of Hebei Province,No.01276134

摘　　要：AIM: To investigate the effects of IH764-3 on HSC apoptosis,and the expression of caspase-3 protein in HSC apoptoticprocess.METHODS: HSCs were cultured in medium with differentIH764-3 doses ( 10 μg@ mL-1 , 20 μg@ mL-1 , 30 μg@ mL-1,40μg @mL-1) and without IH764-3, and HSC proliferation wasquantitatively measured by 3 H-thymidine incorporation. Themorphological changes of HSCs were observed withtransmission electron microscope after exposure to the doseof 40 μg@ mL-1 of IH764-3 for 48 hr, The apoptosis rates weredetected by annexin V/PI and TdT-mediated dUTP nick endlabeling (TUNEL). The expression of caspase-3 protein wasdetermined by flow cytometry.RESULTS: (1) HSC proliferation rates induced with differentIH764-3 doses ( 10 μg@ mL-1 , 20 μg@ mL-1 , 30 μg@ mL-1 , 40 μg@mL-1) were significantly reduced compared with that of thecontrol group ( P＜ 0.01). (2)With the doses above, IH764-3dose-dependently produced HSC apoptosis rates of 6.7 %(9.4%),9.3 %(21.6 %),15.1%(27.2 %) and 19.0 %(28.4 %)respectively, by annexin V and PI-labeled flow cytometry assay(or TUlE L), while it was only 2.3 %(6.7 %) in the control. (3)The expression of caspase-3 protein in IH764-3 groups wassignificantly higher than that of the cortrol (P＜0.05).CONCLUSION: Within the dose range used in present study,IH764-3 can inhibit HSC proliferation, as well as enhance HSCapoptosis. Furthermore, IH764-3 can significantly increasethe caspase-3 protein expression.AIM:To investigate the effects of IH764-3 on HSC apoptosis, and the expression of caspase-3 protein in HSC apoptotic process. METHODS:HSCs were cultured in medium with different IH764-3 doses(10μg·mL^(-1),20μg·mL^(-1),30μg·mL^(-1),40μg· mL^(-1))and without IH764-3,and HSC proliferation was quantitatively measured by ~3H-thymidine incorporation.The morphological changes of HSCs were observed with transmission electron microscope after exposure to the dose of 40μg·mL^(-1)of IH764-3 for 48 hr.The apoptosis rates were detected by annexin V/PI and TdT-mediated dUTP nick end labeling(TUNEL).The expression of caspase-3 protein was determined by flow cytometry. RESULTS:(1)HSC proliferation rates induced with different 1H764-3 doses(10μg·mL^(-1),20μg·mL^(-1),30μg·mL^(-1),40μg· ml^(-1))were significantly reduced compared with that of the control group(P<0.01).(2)With the doses above,IH764-3 dose-dependently produced HSC apoptosis rates of 6.7%(9.4 %),9.3%(21.6%),15.1%(27.2%)and 19.0%(28.4%) respectively,by annexin V and PI-labeled flow cytometry assay (or TUNEL),while it was only 2.3%(6.7%)in the control.(3) The expression of csspase-3 protein in 11-1764-3 groups was significantly higher than that of the control(P<0.05). CONCLUSION:Within the dose range used in present study, IH764-3 can inhibit HSC proliferation,as well as enhance HSC apoptosis.Furthermore,IH764-3 can significantly increase the caspase-3 protein expression.

关 键 词：丹参 肝星状细胞 细胞凋亡 IH764-3 CASPASE-3蛋白 表达 

分 类 号：R285.5[医药卫生—中药学]