含副溶血弧菌(Vibrio parahaemolyticus) Fla I基因真核表达重组质粒的构建  

Construction of Eukaryotic Expression Recombinant Plasmid of Fla I Gene from Vibrio parahaemolyticus

作　　者：周化民[1] 王军[1] 苏永全[1] 丁少雄[1] 鄢庆枇[1] 张朝霞[1] 

机构地区：[1]厦门大学海洋学系亚热带海洋研究所,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2002年第4期498-501,共4页Journal of Xiamen University：Natural Science

基　　金：国家"86 3"计划 (86 3 819 0 2 12 ); 福建省自然科学基金资助项目 (B0 0 10 0 0 4 )

摘　　要：用ClaⅠ /EcoRⅠ双酶切带有副溶血弧菌 (Vibrioparahaemolyticus)极鞭毛蛋白FlaI基因的质粒pMD18 FlaI,回收目的基因片段 ,插入到真核细胞表达载体pIRESneo同样经ClaⅠ /EcoRⅠ切开的窗口中 ,成功地将FlaI克隆到pIRESneo中 ,获得有意义的重组质粒pIRESneo FlaI.构建的pIRESneo FlaI真核表达重组质粒 。The plasmid pMD18 Fla I containing the polar flagellar protein Fla I gene of Vibrio parahaemolyticus was digested with Cla Ⅰ/ Eco RⅠ, and Fla I fragment was recovered and inserted into the eukaryotic expressing vector pIRESneo, which was also digested with Cla Ⅰ/ Eco RⅠ. After ligation with ligase, Fla I was successfully cloned into pIRESneo, and the significant eukaryotic expression recombinant plasmid pIRESneo Fla I was obtained, which will be used for future gene vaccination.

关 键 词：副溶血弧菌 FlaI基因 真核表达载体 鞭毛蛋白基因 NDA免疫 海水鱼类 

分 类 号：Q782[生物学—分子生物学] S941.42[农业科学—水产养殖]