猪笼草的组织培养  

Rapid Propagation of Nepenthes mirabilis by Tissue Culture

作　　者：丰 锋[1] 李洪波[1] 谢建英[1] 

机构地区：[1]湛江海洋大学,广东湛江524088

出　　处：《热带作物学报》2002年第2期62-65,共4页Chinese Journal of Tropical Crops

摘　　要：以猪笼草（Nepenthes mirabilis）腋芽为试材,研究猪笼草的组培快繁。结果表明,外殖体的建立以1/2MS+6-BA 1mg/L+NAA 0.05mg/L液体培养基春季（3～6月）进行培养最佳。愈伤组织（或不定芽）增殖以 MS+6-BA 1mg/L+Ad 10mg/L+NAA 0.1mg/L+100mL/L椰子汁较佳,高浓度（200mL/L）的椰子汁不利于愈伤组织（或不定芽）增殖;继代芽的增殖以MS+6-BA 1.5mg/L+Ad 1～5mg/L+NAA 0.1mg/L+100～200mL/L椰子汁较好:生根培养用1/2MS+6-BA0.1mg/L+NAA1.0mg/L,20d后可长出2～4条根。An experiment was made with axillary buds of nepenthes mirabilis to investigate the optimum conditions for rapid propagation by tissue culture. The results showed that the liquid 1/2MS medium supplemented with 1mg/L 6-BA and 0.05mg/L NAA was optimal for the production of virus-free materials and that the optimal period was from March to May. The optimal medium for proliferation of callus (or adventitious buds) was MS+6-BA1 mg/L+Ad10 mg/L+NAA 0.1 mg/L+ 100 mL/L coconut milk, but high concentration of coconut milk (200mL/L) had inhibitory effect. The optimal medium for subculture was MS+6-BA1.5 mg/L+Ad 1~5 mg/L+NAA 0.1 mg/L+ 100~200 mL/L coconut milk, and 1/2 MS+6-BA0.1 mg/L+NAA 1.0 mg/L for rooting.

关 键 词：猪笼草 组织培养 观叶植物 快繁技术 

分 类 号：S682.36[农业科学—观赏园艺]