金龟子绿僵菌液体深层培养研究  

Submerged Culture of Metarhizium anisopliae

作　　者：宋 漳[1] 

机构地区：[1]福建农林大学林学院,福建南平353001

出　　处：《热带作物学报》2002年第2期72-76,共5页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金资助项目(C96026);福建省教委科学研究基金资助项目(K93036)

摘　　要：研究结果表明：金龟子绿僵菌液生分生孢子的形成与培养基组成密切相关，不同的碳，氮源对液生分生孢子形成具有显著影响，蔗糖是液体深层培养液生分生孢子的理想碳源，而花生饼粉，豆饼粉则是较理想的氮源，且液生分生孢子的产量与培养基氮源的性质有关，复杂的氮源比简单的氮源更有利于液生分生孢子的形成，在供试的8种碳源和6种氮源相互组配的培养液中，以蔗糖+花生饼粉的组合最佳，液生分生孢子产量达14.13±10^9个/L。同时试验结果还表明，液生分生孢子的产出率与培养液的碳氮比，温度，pH有关，在培养温度为28℃，培养液碳氮比为3：1,pH为6.8的条件下，可获得最大的液生分生孢子产量。A strain of Metarhizium anisopliae was incubated in submerged culture. The results showed that the submerged sporulation depended on a delicate equilibrium in the composition of the medium. Carbon and nitrogen sources affected the production of submerged conidia greatly. Sucrose was an ideal carbon source for submerged sporulation. Peanut cake powder and bean cake powder were conducive to sporulation of submerged conidia. The production of submerged conidia was related with the properties of nitrogen sources. Complex nitrogen source was more favorable to sporulation of submerged conidia than simple nitrogen source.Liquid culture medium containing sucrose and peanut cake powder produced a large number of submerged conidia (14.12×109 spores/L). The experiment also showed that the output of submerged conidia was affected by C:N ratio, temperature and pH. The maximum yield of submerged conidia was obtained under the condition of culture temperature at 28℃, C:N ratio at 3:1 and pH 6.8.

关 键 词：金龟子绿僵菌 液体深层培养 菌丝生长 液生分生孢子 昆虫病原真菌 

分 类 号：TQ458[化学工程—农药化工] S476.12[农业科学—农业昆虫与害虫防治]