大鼠趋化素样因子对成肌细胞促增殖作用的研究  

Study on the enhancing effect of rCKLF1, 2 on the proliferation of myoblast cells

作　　者：夏东岚[1] 娄雅欣[1] 韩文玲[1] 李现亭[1] 宋泉声[1] 张颖妹[1] 马大龙[1] 

机构地区：[1]北京大学人类疾病基因研究中心卫生部医学免疫重点实验室,北京100083

出　　处：《细胞与分子免疫学杂志》2002年第4期313-315,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家 8 63高技术计划课题 ;No .2 0 0 1AA2 15 0 61;国家自然科学基金资助 ;No.3 0 0 0 0 15 3

摘　　要：目的　探讨大鼠趋化素样因子 1和 2 (rCKLF1、2 )对成肌细胞的增殖促进作用。方法　构建pcDNA3.1/rCKLF1和pcDNA3.1/rCKLF2真核表达载体 ,并瞬时转染 2 93T细胞 ,收获培养上清。分别以3 H -TdR掺入和MTT比色法 ,分析rCKLF1和rCKLF2对小鼠肌母细胞系C2C12和原代肌卫星细胞增殖的促进作用。结果　成功地构建了rCKLF1和rCKLF2的真核表达质粒 ,并在SV4 0转化的 2 93T细胞中瞬时表达rCKLF1和rCKLF2。瞬时转染上清能够使C2C12细胞的3 H -TdR的掺入值增加 2倍或 3倍。MTT比色法分析表明 ,rCKLF1和rCKLF2的转染上清 ,对Wistar大鼠乳鼠肌卫星细胞具有增殖促进作用。结论　rCKLF1和rCKLF2能够促进骨骼肌细胞的增殖 。Aim To study the effect of rat CKLF1 and CKLF2 (rCKLF1 and rCKLF2)on the proliferation of skeletal muscle cells. Methods The recombinant eukaryotic expression vectors pcDNA3.1/rCKLF1 and pcDNA3.1/rCKLF2 were constructed and transiently transfected into 293T cells. The supernatants were harvested for bioactivity study. Cell proliferation was analyzed by 3 H-TdR incorporation and MTT colorimetry assay. Results We have successfully constructed the eukaryotic expression vectors pcDNA3.1/rCKLF1 and pcDNA3.1/rCKLF2. The supernatants of transfected 293T cells could promote the incorporation of 3 H-TdR into C2C12 cells and MTT uptaking of primary satellite cells. Conclusion Rat CKLF1 and CKLF2 can stimulate proliferation of myoblast cells.

关 键 词：rCKLF 细胞增殖 G2C12细胞 肌卫星细胞 

分 类 号：Q78[生物学—分子生物学] R392－33[医药卫生—免疫学]