小鼠骨髓造血基质细胞体外培养及其功能的研究  

STUDIES ON IN VITRO CULTURE AND FUNCTION OF HEMATO- POIETIC STROMAL CELL FROM MURINE BONE MARROW

作　　者：刘存仁[1] 于善清[1] 贺福初[2] 吴祖泽[2] 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]北京放射医学研究所,北京100850

出　　处：《山东大学学报（理学版）》2002年第2期172-175,共4页Journal of Shandong University(Natural Science)

摘　　要：采用细胞培养技术从正常LACA雄性小鼠骨髓中分离得到 1株造血基质细胞 (HSC) .该细胞胞体较大 ,呈多角形 ,不吞噬酵母多糖 .HSC贴壁层及其培养上清液均具有明显刺激CFU GM体外增殖分化的功能 .将HSC输注到经射线照射的雌性小鼠体内 ,PCR技术检测结果显示 。A strain of hematopoietic stromal cell (HSC)was established from bone marrow in LACA male mouse by using cell culture technique.HSC was found to be large polygonal cells and not to phagocytose zymosan. The conditioned medium and adherent layer of HSC were capable of supporting the proliferation and differentiation of CFU-GM.It was also found that HSC was able to reestablish the hematopoietic microenvironment of murine bone marrow following intravenous injection.

关 键 词：小鼠 骨髓 造血基质细胞 体外培养 功能 细胞培养 增殖分化 造血环境 

分 类 号：Q813.11[生物学—生物工程]