青岛文昌鱼碱性肌球蛋白轻链MLC-alk基因片段的克隆  

CLONING OF QINGDAO AMPHIOXUS ALKALI MYOSIN LIGHT CHAIN GENE FRAGMENT,A MOLECULE INVOLVED IN MUSCULATURE DEVELOPMENT

作　　者：张燕君[1] 梁恺龙[1] 黄向炜[1] 林浴霜[1] 张红卫[1] 

机构地区：[1]山东大学生命科学学院,山东济南250100

出　　处：《山东大学学报（理学版）》2002年第2期176-179,共4页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金 (3 9670 3 5 7;3 0 0 70 0 94) ;高等学校博士学科点专项科研基金

摘　　要：碱性肌球蛋白轻链是构成肌球蛋白头部的必需分子 .采用简并引物PCR方法获得青岛文昌鱼碱性肌球蛋白轻链基因片段 ,并对其氨基酸序列与其他生物如鸡和人的胚胎型、骨骼肌型、平滑肌型、心肌型、非肌型等多种碱性肌球蛋白轻链基因家族成员相应片段进行了同源性分析 ,均显示较高的同源性 .研究结果支持青岛文昌鱼具有 1个 ,可能也仅有 1个碱性肌球蛋白轻链基因 .The alkali myosin light chain genes(MLC alk)code for proteins associated with the globular head of the myosin heavy chain.Degenerate primers were designed according to the conserved sequences of MLC alk gene family and a single MLC alk gene fragment was cloned by PCR from Qindao amphioxus neurula cDNA library.The homology analysis of deduced amino acid sequence showed that Qingdao amphioxus MLC alk had high level of similarities with vertebrate MLC alk genes of embryonic,skeletal muscle,smooth muscle,cardiac muscle and non muscle types.The results show that Qingdao amphioxus contains one and probably only one MLC alk gene.Thus,duplication of an ancestral vertebrate MLC alk gene probably occurred after the vertebrate and amphioxus lineages split.

关 键 词：青岛市 MLC-alk 文昌鱼 碱性肌球蛋白轻链基因 基因克隆 同源性 PCR方法 

分 类 号：Q785[生物学—分子生物学]