大肠埃希菌超广谱β-内酰胺酶的检测及耐药性  

Extended-spectrumβ-lactamase detection and analysis of drug resistance of Escherichia coli

作　　者：赵建平[1] 王文灏[1] 燕成岭[1] 

机构地区：[1]内蒙古自治区医院检验科,呼和浩特010017

出　　处：《江西医学检验》2002年第3期141-142,160,共3页Jiangxi Journal of Medical Laboratory Sciences

基　　金：内蒙古自然基金会资助课题(20001306)部分内容。

摘　　要：目的了解内蒙古地区大肠埃希菌ESBLs的发生率及耐药性,比较三种检测方法。方法纸片扩散初筛法、双纸片协同法、标准纸片扩散确认法。结果大肠埃希菌ESBLs总检出率为10.8%,双纸片协同法与确认法符合率达96.2%,产ESBLs较非ESBLs株耐药性高。ESBLs株的耐药率:IMP为0.0%;PIP/TAZ为4.0%;CFP/SU为20.0%;FOX为24.0%。结论双纸片法简单易行,适合各级医院开展和推广。治疗ESBLs菌的感染,首选碳青霉烯类;对部分β-内酰胺酶抑制剂复合物、头霉烯类有良好的作用。Objective To investigate the incidence of Escherichia coli ESBLs in Inner Mongolia Region and its drug resistance,and to compare three detection methods.Methods Disc diffusion screening method,double-disc synergy method,K-B disc diffu-sion confirmatory method.Results The total detection rate of Escherichia coli ESBLs was10.8%;the coincidence rate between double-disc synergy test and confirmatory test was96.2%,and the drug resistance of ESBLs producing was higher than that of non-ESBLs.The drug resistance of ESBLs was0.0%,4.0%,20.0%,24.0%,respectively to imipenem,piperacillin/tazobactam cefoperaxzone /subactam,cefoxitin.Conclusions The double-disc method is simple and easy,convenient to all kinds of hospitals.To deal with the ESBLs strains,carbapenems are of first choices part ofβ-lactamase inhibitor compounds have beneficial efects.

关 键 词：大肠埃希菌 超广谱Β-内酰胺酶 抗生素 耐药性 纸片扩散初筛法 双纸片协同法 标准纸片扩散确认法 

分 类 号：R446.5[医药卫生—诊断学] R378.21[医药卫生—临床医学]