凝胶过滤层析分离蛋白质实验中三种过滤介质分离效果的比较  

The observation of separating effects on three kinds of gel-filtration chromatography media to separate the protein mixture

作　　者：杨歌德[1] 姜玉梅[1] 周宏博[1] 

机构地区：[1]哈尔滨医科大学生化教研室,黑龙江哈尔滨150086

出　　处：《哈尔滨医科大学学报》2002年第3期242-243,共2页Journal of Harbin Medical University

摘　　要：目的　探讨凝胶过滤层析分离蛋白质的最佳实验效果。方法　分别采用不同规格的凝胶过滤层析介质SephadexG 5 0、G 75、G 10 0 ,通过凝胶过滤层析法分离已知分子量的混合蛋白质。结果　混合样品得到分离 ,呈现三组分离样品的层析图。结论　从大分子物质中除去小分子物质时应选用交联度大的介质G 5 0。进行中等蛋白质分离时 ,选用G 75较为合适。将小分子物质浓缩而除去大分子物质时 ,应选用交联度小的介质 ,如G 10 0或G 2 0 0。Objective To find out the optimal condition for separation of the protein mixture by gel filtration chromatography.Methods Separated the protein mixture of which known molecular relative weight by gel filtration chromatography in sephadex G 50,G 75 and G 100.Results The protein mixture were separated successful lyby gel filtration chromatography and the chromatogram of three separated samples were presented clearly.Conclusion Sephadex G 50 are suitable for purifying the large molecule protein,G 75 for the medium molecule protein,G 100 and G 200 for the small molecule protein.

关 键 词：过滤介质 凝胶过滤层析 葡聚糖凝胶 洗脱液 蛋白质 

分 类 号：R446[医药卫生—诊断学]