氧化苦参碱对哮喘小鼠抗炎作用的研究  

Study on Anti-inflammatory of Oxymatrine on Asthmatic Mice

作　　者：焦霞[1] 沈其昀[1] 王利民[1] 许以平[1] 

机构地区：[1]上海第二医科大学仁济医院呼吸科,上海200001

出　　处：《上海第二医科大学学报》2002年第4期303-305,共3页Acta Universitatis Medicinalis Secondae Shanghai

摘　　要：目的　探讨氧化苦参碱治疗哮喘的抗炎作用机制及其对白细胞介素 -4 (IL -4mRNA )表达的影响。方法　建立小鼠哮喘模型 ,观察氧化苦参碱对哮喘小鼠支气管肺泡灌洗液中炎性细胞、肺组织病理的改变 ;应用半定量反转录 -聚合酶链反应检测肺组织IL -4mRNA表达的变化。　结果　氧化苦参碱可显著减轻哮喘小鼠气道及肺组织中嗜酸性细胞的浸润 ,显著抑制哮喘小鼠肺组织中IL -4mRNA的表达水平。　结论　氧化苦参碱对哮喘小鼠有明显的抗气道变应性炎症及抑制IL -4mRNA表达的作用。Objective To explore the mechanism of oxymatrine anti_inflammatory action and inhibiting IL-4mRNA expression. Methods A murine model of asthma was established to investigate the effects of oxymatrine on the inflammatory cells of bronchoalveolar lavage fluid (BALF) and histologic changes, and IL-4mRNA expression by semiquantitative reverse transcription_polymerase chain reaction (RT-PCR)in asthmatic mice. Results 1.Oxymatrine could significantly reduce the eosinophilic infiltration in BALF and lung tissues in asthmatic mice; 2.Oxymatrine could markedly inhibit IL-4mRNA expression in asthmatic mice lung tissues. Conclusion Oxymatine has significant anti_inflammatory action and can markedly inhibits IL-4mRNA expression in asthmatic mice.

关 键 词：氧化苦参碱 哮喘 抗炎 细胞因子 治疗 

分 类 号：R562.25[医药卫生—呼吸系统]