膜片钳在生命化学中的应用  

Application of Patch-Clamp Technique in Life Chemistry

作　　者：杨频[1] 杜会枝[1] 薛绍武[1] 

机构地区：[1]山西大学分子科学研究所,太原030006

出　　处：《化学进展》2002年第4期251-256,共6页Progress in Chemistry

基　　金：国家自然科学基金重大项目 (批准号 :2 9890 2 80 )

摘　　要：作为先进的电生理技术 ,膜片钳在生命科学研究中已成为一种愈来愈重要的技术。特别是膜片钳与其它生化技术例如 Fura- 2显微荧光测钙技术、单细胞逆转录多聚酶链式反应技术等的有机结合 ,使其应用更加广泛。本文简要叙述了膜片钳的基本原理。As an advanced electrophysiological technique, the patch-clamp technique has become one of the most important methods in the research field of life sciences. It is more powerful when combining with other techniques, such as the fura-2 microfluorometry (for measuring the concentration of Ca2+) and single-cell reverse transcription of RNA followed by the polymerase chain reaction (RT-PCR). In this article, the general mechanism and the models of the patch-clamp technique and its applications to life chemistry are summarized.

关 键 词：生命化学 膜片钳技术 细胞分泌 信号转导 金属粒子 细胞膜行为 分子生物学 离子通道 

分 类 号：Q503[生物学—生物化学]