酒石酸脱除单铽转铁蛋白中铽(Ⅲ)的荧光动力学研究  

Fluorescence Studies on the Kinetics of Terbium(Ⅲ) Removal from Monoterbium Transferrins by Tartaric Acid

作　　者：白海静[1] 刘文[1] 杨斌盛[1] 

机构地区：[1]山西大学分子科学研究所,太原030006

出　　处：《化学学报》2002年第7期1253-1257,共5页Acta Chimica Sinica

基　　金：国家自然科学基金 (No .2 0 0 710 2 2 )资助项目

摘　　要：0 .0 1mol/LHepes,pH 7.4,室温条件下 ,以酒石酸为脱除剂 ,监测铽 (Ⅲ )与脱铁转铁蛋白结合的两种配合物C端单铽转铁蛋白和N端单铽转铁蛋白随酒石酸浓度变化的脱除动力学 ,根据其动力学行为 ,我们推测存在两种平行的脱除途径 :一次途径和饱和途径 ,其中C端单铽转铁蛋白的铽 (Ⅲ )脱除呈现饱和与一次相结合途径 ,N端单铽转铁蛋白为简单的一次途径 .NaCl的加入可促进两种单铽转铁蛋白铽 (Ⅲ )的脱除 。The rates at which terbium(Ⅲ) ion is removed from the two metal binding sites of human serum transferrin by tartaric acid have been evaluated. Solutions are buffered at pH 7.4 by 0.01 mol/L N-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid (Hepes) and maintained at room temperature. According to their kinetic behaviors, it is proposed that there are two parallel removal pathways. One is saturation pathway, the other is first-order pathway. The results show that terbium(Ⅲ) ion removal is through both pathways for the C-terminal site, while through simple first-order pathway for the N-terminal site. The addition of sodium chloride to the buffer accelerates terbium(Ⅲ) ion removal from both forms of monoterbium transferrin, and the C-terminal monoterbium transferrin is more easily affected by sodium chloride.

关 键 词：酒石酸 荧光动力学 人血清转铁蛋白 铽(Ⅲ)离子 荧光光谱 生理功能 氯化钠 

分 类 号：O657.3[理学—分析化学]