血红蛋白在磷脂-月桂酸修饰的玻碳电极上的电化学行为及其分析应用  

Electrochemical Behavior of Hemoglobin at a Lipid-Lauric Acid Modified GC Electrode and Its Application to the Determination of Proteins

作　　者：金松子[1] 王韬[1] 张春煦[1] 沈含熙[1] 

机构地区：[1]南开大学化学学院,天津300071

出　　处：《化学学报》2002年第7期1269-1273,共5页Acta Chimica Sinica

基　　金：国家自然科学基金 (No .2 9875 0 11)资助项目

摘　　要：报道了血红蛋白 (Hb)在磷脂 -月桂酸修饰的玻碳电极上的电化学行为 ,在 +0 .8～ - 0 .7V (vs.Ag/AgCl)电位范围内 ,于pH 6 .0的 0 .0 1mol/L的KH2 PO4 Na2 HPO4 底液中 ,血红蛋白产生不可逆的还原电流峰 .还原峰电流与血红蛋白浓度在 1.2 5× 10 -8～ 4.31× 10 -7mol/L范围内呈良好线性关系 .The electrochemical behavior of hemoglobin at a lipid-lauric acid modified glassy carbon electrode (GC) is reported. In the potential range of + 0.8similar to -0.7 V (vs. Ag/AgCl), an irreversible cathodic peak was observed at -0.4 V at the scan rate of 20 mV/s. In the medium of 0. 01 mol/L KH2PO4-Na2HPO4 (pH 6.0), the cathodic peak current shows linear relationship with the concentration of hemoglobin in the range of 1.25 x 10(-8) similar to 4.31 x 10(-7) mol/L. The electrode can be used as an approach for determining hemoglobin with high sensitivity.

关 键 词：磷脂 月桂酸 电化学行为 分析 血红蛋白 双层类脂膜 磷脂-月桂酸修饰 破碳电极 静电吸附作用 化学修饰电极 

分 类 号：O646[理学—物理化学]