大肠癌及癌旁组织中抑凋亡基因bcl-2和促凋亡基因bax的表达及意义  

Study on the Expression of bcl-2 and bax Gene in Cancerous Tissue and Transitional Mucosa in Colorectal Cancer

作　　者：王举[1] 窦忠霞[2] 韩喜春[3] 程雷[1] 黄大鹤[3] 

机构地区：[1]吉林大学第一医院普通外科,长春130021 [2]内蒙古民族大学附属医院,通辽028000 [3]吉林大学第二医院,长春130021

出　　处：《中国普外基础与临床杂志》2002年第4期252-254,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　研究抑凋亡基因bcl 2和促凋亡基因bax在大肠癌及癌旁组织中的表达及意义。方法　应用免疫组织化学S P法对 3 1例大肠癌患者的癌组织及距癌组织外缘 3cm内的癌旁组织和 3cm以远的正常大肠组织中bcl 2和bax基因的表达进行检测。结果　大肠癌及其癌旁组织中bcl 2蛋白表达阳性率分别为 64 .5 %和 60 .0 % ,显著高于正常组织 (3 5 .0 % ) ,P<0 .0 5 ;bax蛋白表达阳性率分别为 45 .2 %和 45 .0 % ,显著低于正常组织 (60 .0 % ) ,P<0 .0 5 ;大肠癌组织与癌旁组织中bcl 2和bax蛋白表达阳性率的差异无显著性意义 (P>0 .0 5 )。bcl 2和bax蛋白在大肠癌组织中的表达与其病理类型和临床分期无明显关系 (P>0 .0 5 )。结论　大肠癌及其癌旁组织中有不同水平的bcl 2蛋白过度表达和bax蛋白低表达 ,两者通过参与调节细胞凋亡而与大肠癌的发生有关 ,但与大肠癌的病理类型、临床分期无关。距大肠癌组织外缘 3cm以内的癌旁组织应视为癌前期组织 。Objective To study the expression of apoptosis suppressing gene (bcl 2) and apoptosis promoting gene (bax) in colorectal cancerous tissue and transitional mucosas. Methods Colorectal cancerous tissue, transitional mucosas (3 cm from the cancerous tissue) and normal tissue were taken respectively in thirty one cases. Immunohistochemical technique S P method was used to detect the expression in those tissues. Results The positive expression rate of bcl 2 protein in cancerous tissue and transitional mucosa were 64.5%and 60.0% respectively and significantly higher than that in normal tissue ( P <0.05). The positive expression rate of bcl 2 protein in normal tissue was 35.0%. The positive expression rate of bax protein in cancerous tissue and transitional mucosa were 45.2%and 45.0% respectively and significantly lower than that in normal mucosa ( P <0.05). The positive expression rate of bax protein in normal tissue was 60.0%. There was no obvious difference in the positive rate of bax and bcl 2 protein between cancerous tissue and transitional mucosa ( P >0.05) . The expression rate of bax and bcl 2 protein in colorectal cancer was irrelative to clinical pathological gradation and clinical stage ( P >0.05). Conclusion There is over expression of bcl 2 protein and low expression of bax protein in colorectal cancer and transitional mucosa. bcl 2 protein and bax protein can affect the generation of colorectal cancer by participating in the regulation of apoptosis. But it is irrelative to clinical pathological gradation and clinical stage. Transitional mucosas should be viewed as precancerous lesion and resected during operation.

关 键 词：抑凋亡基因 促凋亡基因 表达 大肠癌 癌旁组织 bcl-2基因 BAX基因 细胞凋亡 

分 类 号：R735.34[医药卫生—肿瘤]