电镜放射自显影技术在内耳病理研究中的应用  

Electron Microscopic Autoradiography in Inner Ear Pathological Study

作　　者：聂国辉[1] 汪吉宝[2] 

机构地区：[1]深圳市红十字会医院耳鼻咽喉科,深圳518035 [2]华中科技大学同济医学院附属协和医院耳鼻咽喉科

出　　处：《听力学及言语疾病杂志》2002年第3期181-182,T002,共3页Journal of Audiology and Speech Pathology

基　　金：卫生部科学研究基金资助项目 (编号 96 114 6 )

摘　　要：目的　研究电镜放射自显影技术在内耳病理研究中的应用。方法　将有放射活性的小鸡内耳组织切成超薄切片 ,制成电镜观察标本 ,涂乳胶膜后通过显影、定影 ,电镜下观察标记的增殖细胞。结果　标记细胞中银颗粒准确定位于细胞核上 ,细胞核结构清晰可见。Objective To evaluate the technique of electron microscopic autoradiography in inner ear pathology.Methods The thin sections that was labeled with the thymidine-H3(3H-TdR)were prepared.The labeled specimen were processed for electron microscopic autoradiography(EM-ARG)after treatment with emulsions,development and fixation.Results Incorporation of 3H-TdR was seen over regenerated cells in the experimental papilla in regions of hair cell loss.Conclusion This methocl gives a good localization of the label at the ultrastructural level.

关 键 词：电镱放射自显影技术 内耳 病理 EM-ARG 动物实验 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]