利福霉素B发酵放大 Ⅰ.从摇瓶到15L发酵罐的发酵放大  

Scale-up of rifamycin B fermentation Ⅰ.Scale-up of rifamycin B fermentation from shaking flask to 15L fermenter

作　　者：赵成建[1] 张定丰[1] 金志华[2] 

机构地区：[1]浙江医药股份有限公司新昌制药厂,新昌312500 [2]浙江大学材料与化工学院生物工程研究所,杭州310027

出　　处：《中国抗生素杂志》2002年第7期398-400,430,共4页Chinese Journal of Antibiotics

摘　　要：研究了溶氧和剪切力对地中海拟分枝酸菌 XC- 92 5发酵产生利福霉素 B的影响 ,并对利福霉素B从摇瓶到 15 L 发酵罐的发酵放大及 15 L 发酵罐流加补料发酵进行了研究。利用摇瓶研究结果表明地中海拟分枝酸菌 XC- 92 5发酵产生利福霉素 B时对溶氧要求较高 ,而对剪切力不太敏感。以溶氧为依据 ,在 15 L发酵罐间歇发酵过程中 ,控制溶氧不低于 2 5 % ,利福霉素 B的发酵效价可达到 10 0 0 0 u/ ml左右 ,发酵周期 (6 d)较摇瓶发酵缩短了 1d。 15 L 发酵罐如采用流加补料发酵工艺 ,利福霉素 B的发酵效价还会有较大幅度提高。The effect of dissolved oxygen and other parameters of rifamycin B in shaking flask experiments and the scale up fermentation,as well as with continuous feeding process in 15L fermenter were studied. Results showed that by controlling the dissolved oxygen content higher than 25% (v/v) in a 6 day bath fermentation, the potency of rifamycin B in 15L fermenter reached above 10000u/ml; while using continuous feeding process with glucose and ammomia water,the potency could be increased significantly.

关 键 词：利福霉素B 地中海拟分枝酸菌 发酵放大 抗生素 

分 类 号：R978.1[医药卫生—药品] TQ465.9[医药卫生—药学]