高效毛细管电泳法测定八珍丸和八珍益母丸中芍药苷的含量  

Determination of Paeoniflorin in Bazhen Pills and Bazhen Yimu Pills by High Performance Capillary Electrophoresis(HPCE)

作　　者：陈勇川[1] 朱卫民[2] 刘松青[1] 

机构地区：[1]第三军医大学西南医院药学部,重庆市400038 [2]重庆医科大学附属第一医院,重庆市400016

出　　处：《中国药房》2002年第7期426-427,共2页China Pharmacy

摘　　要：目的 :建立高效毛细管电泳法测定八珍丸和八珍益母丸中芍药苷的含量方法。方法 :运行缓冲液为50mmol/L硼砂缓冲液(pH10 6) ;操作电压为20KV ;检测波长为230nm。结果 :测定芍药苷的线性范围为0 0625～1 0mg/ml,r=0 9995;加样回收率为98 19%～98 45% ;日内和日间精密度分别为1 61%和3 15 %。测定了八珍丸和八珍益母丸中芍药苷的含量 ,前者结果与高效液相色谱法一致。结论 :该分析方法简便、快速、经济。OBJECTIVE:A HPCE method for determination of paeoniflorin in Bazhen pills and Bazhen Yimu pills was developed METHODS:Measurements were carried out in a buffer solution containing 50mmol/L borax solution (pH 10 6),at detection wavelength 230nm with 20KV running voltage applied to a 50cm×50μm capillary RESULTS:The linear range of paeoniflorin was in the range of 0 0 625～1 0mg/ml(r=0 9 995),RSD within days was 1 61%,RSD between days was 3 15% The recovery of paeoniflorin was 98 19%～98 45% The results of detection for Bazhen pills and Bazhen Yimu pills were highly correlated to those obtained by HPLC CONCLUSION:The method is simple,rapid,economical and accurate

关 键 词：高效毛细管电泳法 芍药苷 八珍丸 八珍益母丸 含量测定 

分 类 号：R284.1[医药卫生—中药学]