电针对面神经再生逆行轴突转运神经营养因子的影响  

Effects of Electroacupuncture on Neurotrophins Responsible for Facial Nerve Regeneration in the Rabbit

作　　者：吴滨[1] 何竟[1] 高炳庆[1] 李春华[1] 刘屹[1] 

机构地区：[1]四川大学华西医院,成都610041

出　　处：《针刺研究》2002年第2期108-111,共4页Acupuncture Research

基　　金：四川省中医药管理局资助课题 (NO .9816 )

摘　　要：目的 :了解电针对面神经逆行轴突转运神经营养因子的影响。方法 :同龄成年健康的新西兰兔 ,均在 3 %的戊巴比妥钠静脉麻醉下 ,分离暴露面神经上颊支 ,在手术放大镜下切断神经 ,用硅胶管将两断端嵌入并缝合固定 ,形成再生室。术后动物随机分三组 ,分别接受1 2 5I BDNF ,1 2 5I NT 3或1 2 5I NT 4再生室内注射 ,1 0 μL/只。每组又随机分为针刺组和对照组。针刺组于术后2hr和 2 6hr各接受 1次电针治疗 ,对照组不作任何处理。术后 4hr、8hr、1 2hr、2 4hr、48hr摘取含面神经核的脑干检测 ,两组对照。结果 :对照组脑干中1 2 5I BDNF ,1 2 5I NT 3 ,或1 2 5I NT 4的γ计数在1 2hr和 2 4hr达到峰值 ,48hr又明显降低 ,不同时段波动很大。而针刺组脑干的1 2 5I BDNF ,1 2 5I NT 3或1 2 5I NT 4γ计数在各个时段始终保持在较高的水平。结论Objective:To observe the effect of electroacupuncture (EA) on neurotrophins responsible for facial nerve regeneration in the rabbit. Methods: Under 3% pentobarbital anesthesia (30 mg/kg), a surgical operation was performed in 180 adult New Zealand rabbits by separating and sectioning the buccal branch of the facial nerve trunk and implanting a silicone tube (forming a regeneration chamber) for placing two cut ends of the nerve. All the animals were treated by injecting 125I-BDNF (brain derived neurotrophic factor), 125I-NT(neurotrophin)-3 and 125I-NT-4 (each one being 10 μL) into the chamber respectively. Rabbits treated with the 3 types of NT were randomized into control and EA groups separately. 'Yifeng'(TE 17), 'Dicang'(ST 4), 'Jiache' (ST 6) and 'Hegu' (LI 4) were punctured and stimulated electrically with frequency of 18～20 Hz, strength of 1.5 V and sparse-dense waves for 30 min. 4 hr, 8 hr, 12 hr, 24 hr and 48 hr after operation, each 6 animals of the two groups in every time-course were killed for taking out the brain stem samples. 125I-BDNF, 125I-NT-3 and 125I-NT-4 radioactivity of the brain stem tissues was detected with γ-counter respectively. Results: In control group, 125I-BDNF, 125I-NT-3 and 125I-NT-4 count values peaked from 12 hr to 24 hr following operation, while in treatment group, these neurotrophin values in all the time-courses kept in a higher level. Significant differences were found between EA and control groups in different time-courses in the 3 indexes (P<0.01～0.001). Conclusion: Electroacupuncture may facilitate the retrograde transport of the neurotrophins in the facial nerve, suggesting a promoting action of EA on nerve regeneration.

关 键 词：电针 脑干神经营养因子 逆行轴突转运 

分 类 号：R245.97[医药卫生—针灸推拿学]