腧穴组方对急性胃粘膜损伤大鼠胃粘膜形态学的影响  

Effects of Different Acupoint Recipes on Morphological Changes of Gastric Mucosa in Rats with Acute Gastric Injury

作　　者：冀来喜[1] 燕平[1] 郝重耀[1] 阎丽萍[1] 杨恩来[1] 

机构地区：[1]山西中医学院针灸系,太原030024

出　　处：《针刺研究》2002年第2期136-139,135,共5页Acupuncture Research

摘　　要：目的 :探讨腧穴不同组方对急性胃粘膜损伤大鼠胃粘膜形态学的影响。方法 :以乙醇造成大鼠急性胃粘膜损伤后 ,动态观察针刺“足三里”不同组穴对模型胃粘膜组织学及超微结构的影响。结果 :①“足三里”不同穴组对急性胃粘膜损伤均具有保护作用。②在不同穴组中 ,以“足三里” +“内关” +“中脘”组效果最好。结论 :“足三里” +“内关” +“中脘”Objective: To observe the effect of different prescriptions of acupoints on morphological changes in rats with experimental acute injury of gastric mucosa(AIGM). Methods: 72 Wistar rats anesthetized with pentobarbital (40～50 mg/kg) were randomized into control group (n=6), model group, 'Zusanli' group, 'Zusanli'+'Neiguan' group, 'Zusanli' +'Zhongwan' group and 'Zusanli' +'Neiguan' +'Zhongwan' group. The last 5 groups were further divided into immediate subgroups, gastric samples were taken immediately after electroacupuncture (EA) treatment and 8 hr-subgroups (samples were taken 8 hours after EA), with 6 cases being in each subgroup. Acute gastric injury model was established by intragastric perfusion of absolute ethyl alcohol (1 mL). Bilateral 'Zusanli' (ST 36), bilateral 'Neiguan' (PC 6) and 'Zhongwan' (CV 12) were punctured and stimulated electrically with frequency 30 Hz, 10～20 V and duration of 10 min. At the end of each experiment, the rat was killed for taking out the whole stomach that was embedded with paraffin and then sectioned and stained with HE. The sections of the stomach were observed under light microscope and electronic microscope for analyzing changes of the injured gastric mucosa. Results: ① After administration of alcohol, gastric mucosal injury was apparent in every subgroups compared with control group, but the injury degrees in every acupuncture subgroup were significantly lighter than that of model subgroups (P<0.05～0.001). ② Among the different acupoint prescription groups, the injured severity of 'Zusanli' +'Neiguan' +'Zhongwan' group was lightest under both light microscope and electronic microscope (no obvious swelling of the mitochondria, regular arrangement of the mitochondria crest and the endoplasmic reticulum without dilation). Conclusion: Acupuncture treatment has a protective action on alcohol induced injury of the gastric mucosa, and the prescription of 'Zusanli' +'Neiguan' +'Zhongwan' may be used as the basic prescription for treating gastric diseases in c

关 键 词：急性胃粘膜损伤 针刺治疗 组织学 超微结构 

分 类 号：R246.1[医药卫生—针灸推拿学] R245－0[医药卫生—中医临床基础]